Composite reagent for fast preparation of portal hypertension animal models and preparation process

Abstract

Composite reagent for a fast preparation of portal hypertension animal models is composed of granulated medical grade polyurethane, analytical pure tetrahydrofuran, and tetrachloromethane solution. A preparation process includes the following steps: (1) cleaning and drying the granulated medical grade polyurethane and placing into a sealable glass container, (2) adding the analytical pure tetrahydrofuran to the glass container, sealing the container, and placing the container still at a room temperature, (3) adding the tetrachloromethane solution, shaking and mixing evenly, (4) puncturing a portal vein under guide of B-ultrasound or injecting the composite reagent at a time by puncturing the portal vein on a hypogastric small incision, and therefore the portal hypertension animal models are built instantly. As organic solvent in the composite reagent is compatible with water in blood, polyurethane constituents are rapidly separated out to prevent pressure of the portal vein from rising due to blood flow of the portal vein. Meanwhile, tetrachloromethane enables liver to become cirrhosis so that the increasing pressure of the portal vein is stabilized. The composite reagent for the fast preparation of the portal hypertension animal models and the preparation process have the advantages of being fast in speed, little in damage, simple to operate and high in success rate.

Description

technical field [0001] The invention relates to a reagent for preparing an animal model, in particular to a compound reagent for rapidly preparing an animal model of canine portal hypertension and a preparation process. Background technique [0002] Portal hypertension is a common clinical disease, and the evaluation of the etiology, pathogenesis, related treatment measures and efficacy of portal hypertension cannot be separated from animal experiments, and the premise of animal experiments is to establish animal models. At present, the reagents used in animal models of portal hypertension are mainly hepatotoxic reagents and carcinogens, such as carbon tetrachloride, dimethylnitrosamine, diethylnitrosamine, and thioacetamide. When these reagents are used to prepare animal models of dog portal hypertension, small doses, long-term, and multiple subcutaneous injections or intragastric administration are required. The disadvantages are that the model establishment period is long...

AI Technical Summary

Problems solved by technology

When these reagents are used to prepare animal models of dog portal hypertension, small doses, long-term, and multiple subcutaneous injections or intragastric administration are required. The disadvantages are that the model establishment period is long and the animal mortality rate is high. In addition, repeated use of these reagents is very easy to cause environmental pollution

Therefore, existing reagents that are used to establish animal models of canine portal hypertension have various defects, which seriously affect the development of relevant scientific research.