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Application of DCX-SPRAC double-gene co-expression vector in preparation of radiotherapy sensitization agent

A co-expression vector, dual-gene technology, applied in gene therapy, application, genetic engineering, etc., can solve the problem of glioma radiotherapy insensitivity, and achieve the effect of improving radiosensitivity and expression level

Inactive Publication Date: 2012-12-26
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a DCX and SPARC dual gene co-expression vector in the preparation of radiotherapy sensitizers, to solve the problem of glioma insensitivity to radiotherapy

Method used

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  • Application of DCX-SPRAC double-gene co-expression vector in preparation of radiotherapy sensitization agent
  • Application of DCX-SPRAC double-gene co-expression vector in preparation of radiotherapy sensitization agent
  • Application of DCX-SPRAC double-gene co-expression vector in preparation of radiotherapy sensitization agent

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Experimental program
Comparison scheme
Effect test

Embodiment

[0034] one. Construction of recombinant adenoviral vector for co-expression of DCX and SPARC genes

[0035] technical process

[0036] 1. Design primers for DCX and SPARC (Table 1), introduce BglII and KpnI restriction sites at both ends of DCX, and introduce NotI and XhoI restriction sites at both ends of SPARC. Using the human cDNA library as a template, the target fragments of DCX and SPARC were amplified under the action of Phusion high-fidelity DNA polymerase, and identified by agarose electrophoresis.

[0037] Table 1. PCR Primers

[0038]

[0039] 2. Double-digest the PCR product of the target gene and the pAdTrack-CMV-polyA-promoter empty vector transfer plasmid, and use T4 DNA ligase to ligate it overnight at 4°C (for the connection method, see figure 1 ). The ligation product was transformed into DH5α Escherichia coli, and LB agar plate (containing 50 μg / ml kanamycin) was used to screen for resistant clones. After the DNA sequence was determined, the positive...

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Abstract

The invention discloses the application of a DCX-SPRAC double-gene co-expression vector in a preparation of radiotherapy sensitization agent. By constructing the DCX-SPRAC double-gene co-expression recombinant adenovirus vector, the radiotherapy sensitivity of glioma can be improved effectively.

Description

technical field [0001] The invention relates to a gene medicine, in particular to the application of DCX and SPARC double-gene co-expression vectors in the preparation of radiotherapy sensitizers. Background technique [0002] Glioma is a tumor that occurs in the neuroectoderm, so it is also called neuroectodermal tumor or neuroepithelial tumor. [0003] The basic treatment for glioma is surgical resection + radiotherapy and chemotherapy. With the development of molecular biology, immunobiology, tumor immunology and medical bioengineering, new methods and technologies for the treatment of glioma are emerging, such as: cellular immunotherapy, molecular targeted therapy, hyperthermia, etc. Among these treatment methods, each has its own limitations. [0004] Glioma is not very sensitive to radiotherapy because of its malignancy, and it is generally believed that poorly differentiated tumors are higher than well-differentiated tumors. The clinical effect of radiotherapy is n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00C12N15/861C12N15/66A61P35/00
Inventor 刘芬菊徐媛媛蒋昕俞家华
Owner SUZHOU UNIV
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