SiRNA inhibiting PPFIA1 gene expression, and its application

A gene expression and gene technology, applied in the field of siRNA inhibiting PPFIA1 gene expression

Active Publication Date: 2012-12-19
广州安镝声生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in chronic myelogenous leukemia, there is no research report on the PPFIA1 gene

Method used

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  • SiRNA inhibiting PPFIA1 gene expression, and its application
  • SiRNA inhibiting PPFIA1 gene expression, and its application
  • SiRNA inhibiting PPFIA1 gene expression, and its application

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Experimental program
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Effect test

Embodiment 1

[0030] (1) Design and synthesis of PPFIA1siRNA:

[0031] According to the known sequence of PPFIA1 in the GenBank gene bank (Gene ID: 8500, NM_003626.2, NM_177423.1), according to the siRNA design principle, combined with the online design tool siCatch provided by Ambion TM siRNA Design is designed for the siRNA target position in the coding region;

[0032] The sequence of PPFIA1 siRNA is:

[0033] Sense strand: 5'-CCACAAAGCUCUGGAUGAA-3',

[0034] Antisense strand: 5'-UUCAUCCAGAGCUUUGUGG-3';

[0035] There is dTdT overhanging at the 3' end of the PFIA1 siRNA sequence, which was synthesized by Guangzhou Ruibo Biotechnology Co., Ltd.

[0036] The random sequence RNA duplex (scramble, SCR) was used as a random control, and the random sequence RNA duplex was purchased from Shanghai Gemma Pharmaceutical Technology Co., Ltd., and its catalog number is: A06001.

[0037] (2) Culture of leukemia K562 cells

[0038] Leukemic K562 cells were inoculated in 1640 medium containing 10...

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Abstract

The invention discloses an siRNA inhibiting the PPFIA1 gene expression, and its application. The positive-sense strand sequence of the siRNA is 5'-CCACAAAGCUCUGGAUGAA-3', the antisense strand sequence of the siRNA is 5'-UUCAUCCAGAGCUUUGUGG-3', and dTdT is dangled at the 3' segment of the siRNA seuqnce. The siRNA inhibiting the PPFIA1 gene expression can effectively silence the PPFIA1 gene and provides a technical scheme for researches on the functions of the PPFIA1 gene, and the silenced PPFIA1 gene can inhibit the cell growth and promote the cell apoptosis; and the siRNA can be used for preparing anti-leukemia medicines.

Description

technical field [0001] The invention relates to an siRNA, in particular to an siRNA for inhibiting the expression of PPFIA1 gene and its application. Background technique [0002] Chronic myelogenous leukemia (CML) is a malignant proliferative disease of hematopoietic stem cells mainly characterized by myelogenous hyperplasia, and its characteristic cytogenetic marker is Ph chromosome t(9;22)(q34;q11 ). P210BCR-ABL fusion protein is the product of Ph chromosome, has strong tyrosine kinase activity, and is closely related to the occurrence of CML. Its production can lead to the continuous phosphorylation of a series of signaling proteins downstream of it, thereby causing hematopoietic stem cells Pathophysiological changes such as malignant proliferation, anti-apoptosis, and adhesion function defects occur. Epidemiological surveys show that the incidence of CML among leukemia in my country is 1 / 10 5 , second only to Jili and Jilin, ranking third, showing a clear increasing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/68C12Q1/02G01N33/68A61K48/00A61P35/02
Inventor 费嘉朱雪娇骆小闯李育敏
Owner 广州安镝声生物医药科技有限公司
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