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High-yield polymalic acid-producing bacterial strain, application and method thereof

A polymalic acid, high-yield technology, applied in the field of fermentation, can solve the problems of high raw material price, low polymalic acid yield, low utilization efficiency, etc., and achieve the effect of low raw material cost, low price and easy extraction

Active Publication Date: 2012-12-19
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]At present, polymalic acid is mainly produced by fermentation of Aureobasidium pullulans, mainly using starch and glucose as raw materials. High price limits industrial applications to a certain extent
Molasses is the main by-product of the sugar industry. The molasses is mainly composed of sucrose, which can be utilized by Aureobasidium pullulans, but the utilization efficiency is low, and the yield of polymalic acid is low, so it cannot be applied on a large scale in industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method for producing polymalic acid, the concrete steps are:

[0034] a. seed activation

[0035] Inoculate Aureobasidium pullulans FMT1801 CCTCC NO: M 2012223 in the seed medium, the inoculation amount is 1:100 according to the volume ratio of Aureobasidium pullulans FMT1801 seed solution to the seed medium, and the formula of the seed medium is glucose 60g / L, Ammonium nitrate 2 g / L, KH 2 PO 4 0.05g / L, ZnSO 4 0.05g / L, MgSO 4 0.05g / L, corn steep liquor 0.05g / L and CaCO 3 20 g / L was shaken for 48 hours at a temperature of 25°C and a rotational speed of 250 rpm to obtain an activated seed solution.

[0036] b. Fermentation

[0037] The activated seed liquid obtained in step a is inoculated in the shake flask containing 50mL fermentation medium, the inoculum is 1:10 according to the volume ratio of the activated seed liquid and the fermentation medium, and the fermentation medium formula is molasses 100 g / L, potassium nitrate 2 g / L, KH 2 PO 4 0.05g / L, Zn...

Embodiment 2

[0042] The method for producing polymalic acid, the concrete steps are:

[0043] a. seed activation

[0044] Inoculate Aureobasidium pullulans FMT1801 CCTCC NO: M 2012223 in the seed medium, the inoculation amount is 1:100 according to the volume ratio of Aureobasidium pullulans FMT1801 seed solution to the seed medium, and the formula of the seed medium is glucose 80g / L, Ammonium nitrate 4 g / L, KH 2 PO 4 0.2g / L, ZnSO 4 0.2g / L, MgSO 4 0.2g / L, corn steep liquor 2g / L and CaCO 3 40 g / L was shaken for 96 hours at a temperature of 25°C and a rotational speed of 180 rpm to obtain an activated seed solution.

[0045] b. Fermentation

[0046] The activated seed liquid obtained in step a is inoculated in the shake flask containing 50mL fermentation medium, the inoculum is 1:10 according to the volume ratio of the activated seed liquid and the fermentation medium, and the fermentation medium formula is molasses 150g / L, sodium nitrate 4 g / L, KH 2 PO 4 0.2g / L, ZnSO 4 0.2g...

Embodiment 3

[0051] The method for producing polymalic acid, the concrete steps are:

[0052] a. seed activation

[0053] Inoculate Aureobasidium pullulans FMT1801 CCTCC NO: M 2012223 into the seed medium, the inoculation amount is 1:100 according to the volume ratio of Aureobasidium pullulans FMT1801 seed liquid to seed medium, and the formula of the seed medium is glucose 100 g / L , ammonium nitrate 5 g / L, KH 2 PO 4 0.3g / L, ZnSO 4 0.3g / L, MgSO 4 0.3g / L, corn steep liquor 3 g / L and CaCO 3 50g / L was shaken for 36 hours at a temperature of 28°C and a rotational speed of 300 rpm to obtain an activated seed solution.

[0054] b. Fermentation

[0055] The activated seed liquid obtained in step a is inoculated in the shake flask containing 50mL fermentation medium, the inoculum is 1:10 according to the volume ratio of the activated seed liquid and the fermentation medium, and the fermentation medium formula is molasses 200 g / L, ammonium nitrate 5 g / L, KH 2 PO 4 0.3g / L, ZnSO 4 0....

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PUM

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Abstract

The invention discloses high-yield polymalic acid-producing bacterial strain, which specifically is aureobasidium pullulans FMT1801, wherein the preservation number is CCTCC (China Center For Type Culture Collection) NO: M2012223; industrial by-product honey can be adopted as raw material; the material cost is low, and the yield of polymalic acid is high. The invention also discloses application of the bacterial strain in production of polymalic acid and a method for producing polymalic acid. The bacterial strain has the advantages of simple process, low production cost and wide application prospect.

Description

[0001] technical field [0002] The invention relates to the field of fermentation, in particular to a bacterial strain with high yield of polymalic acid, and also relates to the application and method of the bacterial strain. Background technique [0003] Polymalic acid (PMLA) is polymerized in vivo with L-malic acid as the only monomer. It is a new type of fully biodegradable polymer material with high biocompatibility, biodegradability and biodegradability. Absorbency and other advantages. Because PMLA has a pendant carboxyl group on the side chain, it is easy to react with other functional groups to prepare PMLA derivatives. Compared with materials such as polylactic acid, PMLA shows better advantages in terms of easy metabolism and modification in vivo, and is used in medicine. It has broad application prospects in preparation, food packaging, agriculture and other aspects. Polymalic acid can also be hydrolyzed into a monomer, that is, L-malic acid, also known as 2-hy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/62C12R1/645
Inventor 邹祥戢运超
Owner SOUTHWEST UNIV
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