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Universal hapten of fluoroquinolone medicines, artificial antigen, broad-spectrum monoclonal antibody, preparation method and application

A fluoroquinolone and monoclonal antibody technology, applied in the field of food safety, can solve the problem that antibodies cannot recognize fluoroquinolone drugs, etc., and achieve the effect of satisfying on-site rapid detection, reducing detection cost, and improving detection efficiency

Inactive Publication Date: 2012-12-19
河北英茂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing literature reports use the carboxyl group in a certain fluoroquinolone drug molecule to directly link with the carrier protein as an immunogen to prepare antibodies, and the obtained antibodies cannot recognize all fluoroquinolone drugs

Method used

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  • Universal hapten of fluoroquinolone medicines, artificial antigen, broad-spectrum monoclonal antibody, preparation method and application
  • Universal hapten of fluoroquinolone medicines, artificial antigen, broad-spectrum monoclonal antibody, preparation method and application
  • Universal hapten of fluoroquinolone medicines, artificial antigen, broad-spectrum monoclonal antibody, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Experiment 1: Synthesis of a universal hapten for fluoroquinolones

[0038] (a) In a 50ml three-neck flask, add 8ml of distilled water, heat to 80°C, add 1mmol of sarafloxacin standard substance into the bottle, and add dropwise sodium hydroxide solution until dissolved, slowly add 1mmol of 2- Chloroethylamine, stirred slowly for 2 hours, during which sodium hydroxide solution was continued dropwise to maintain pH=8.0;

[0039] (b) Lower the temperature of the solution in step (a) to 60°C, add hydrochloric acid to neutralize to pH=6.0, and precipitate a solid, suction filter the obtained solid, and wash with water to obtain the sarafloxacin hapten. The molecular structural formula of product is as shown in formula 1:

[0040]

[0041] Formula 1.

[0042] The product has a melting point of 225°C; infrared spectrum (IR) characterization data: (KBr)V max 3600, 3392, 3075, 2975, 2825, 1733, 1627, 1490, 1259, 1157, 806, 730, 540cm -1 . Compared with the infrared data...

Embodiment 2

[0051] Experiment 1: Synthesis of a universal hapten for fluoroquinolones

[0052] (a) In a 50ml three-neck flask, add 8ml of distilled water, heat to 75°C, add 1mmol of sarafloxacin standard substance into the bottle, and add dropwise sodium hydroxide solution until dissolved, slowly add 1.5mmol of 2 - Chloroethylamine, stirred slowly for 2.5 hours, during which sodium hydroxide solution was continued dropwise to maintain pH=7.5;

[0053] (b) Lower the temperature of the solution in step (a) to 55°C, add hydrochloric acid to neutralize to pH=5.5, and precipitate a solid, suction filter the resulting solid, and wash with water to obtain the sarafloxacin hapten. The molecular structural formula of product is as shown in formula 1:

[0054]

[0055] Formula 1.

[0056] The product structure and characterization data are the same as above.

[0057] Experiment 2: Preparation of common artificial antigens for fluoroquinolones

[0058] (a) 60 mmol of sarafloxacin hapten was p...

Embodiment 3

[0065] Experiment 1: Synthesis of a universal hapten for fluoroquinolones

[0066] (a) In a 50ml three-neck flask, add 8ml of distilled water, heat to 85°C, add 1mmol of sarafloxacin standard substance into the bottle, and add dropwise sodium hydroxide solution until dissolved, slowly add 2mmol of 2- Chloroethylamine, stirred slowly for 3 hours, during which sodium hydroxide solution was continued dropwise to maintain pH=8.5;

[0067] (b) Lower the temperature of the solution in step (a) to 65°C, add hydrochloric acid to neutralize to pH=6.5, and precipitate a solid, suction filter the resulting solid, and wash with water to obtain the sarafloxacin hapten. The molecular structural formula of product is as shown in formula 1:

[0068]

[0069] Formula 1.

[0070] The product structure and characterization data are the same as above.

[0071] Experiment 2: Preparation of common artificial antigens for fluoroquinolones

[0072] (a) 50 mmol of sarafloxacin hapten was placed...

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Abstract

The invention discloses a universal hapten of fluoroquinolone medicines, artificial antigen, a broad-spectrum monoclonal antibody, a preparation method and an application, which designs and synthesizes the universal hapten containing a fluoroquinolone medicine mother nucleus structure, the artificial antigen, and the broad-spectrum monoclonal antibody capable of identifying various fluoroquinolone medicines. The universal hapten of fluoroquinolone medicines, the artificial antigen and the broad-spectrum monoclonal antibody can be used for immunoassay and screening of the fluoroquinolone medicines, the detection efficiency is enhanced, the detection time is shortened and the detection cost can be reduced.

Description

technical field [0001] The invention relates to the field of food safety, in particular to a general hapten, an artificial antigen and a broad-spectrum monoclonal antibody of fluoroquinolone drugs, and a preparation method and application thereof. Background technique [0002] The third-generation quinolones are a class of synthetic antibacterial drugs with a basic structure of 6-fluoro-7-piperazine-4-quinolone ring, also known as fluoroquinolones. Fluoroquinolones have low toxicity, long half-life, broad antibacterial spectrum and strong antibacterial activity, and are widely used in the treatment of various infectious diseases in animals and humans. Sarafloxacin is a typical representative of fluoroquinolones. [0003] With the widespread application of fluoroquinolones in food animals, their residues in animal-derived foods have also attracted widespread attention. The residues of fluoroquinolones are not only directly harmful to human health due to their toxic and side...

Claims

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Application Information

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IPC IPC(8): C07D215/56C07K14/765C07K14/77C07K1/113C07K16/44G01N33/577
Inventor 赵国先王建平刘彦政刘静张会彩
Owner 河北英茂生物科技有限公司
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