Micro-nano driving platform for real-time variable-frequency regulation and control of muscle fiber

Abstract

The invention discloses a micro-nano driving platform for real-time variable-frequency regulation and control of muscle fibers, which comprises a central processing module, three experiment groove modules with a same structure, a motor servo platform module, a microscopic observation module, a data processing module and an environment regulation and control module, wherein the central processing module is connected with the three experiment groove modules, the motor servo platform module, the microscopic observation module, and the data processing module; the data processing module is connected with the microscopic observation module; the environment regulation and control module is respectively connected with the three experiment groove modules; the microscopic observation module is respectively connected with the three experiment groove modules. The platform of the invention is compact in structure, durable, and stable, has the function of stimulation pulse variable-frequency transmission, breaks through the limitation of single freedom degree for routine muscle fiber experiment platforms, can be used for muscle fiber complex motion control experiments in a two dimensional plane, and provides experiment means for the exploration of muscle control principles and the further verification of 4M models.

Description

technical field [0001] The present invention relates to a general-purpose two-dimensional bioelectrochemical / mechanical experiment platform for animal muscle cells (muscle fibers), in particular to a micro-nano drive platform for real-time frequency conversion regulation of muscle fibers, which is aimed at the exploration of the principles of muscle fiber operation control and the Validation of the muscle 4M model. Background technique [0002] The research on the operating mechanism of skeletal muscle has been carried out for many years at home and abroad. Because of its important value to physiology, life science and rehabilitation science, it has always been a frontier issue and research hotspot in the academic circle. From the current point of view, relevant research is mainly carried out on the following three scales: 1) From the microscopic level, the research mainly focuses on the interaction mechanism between myosin (molecular motor) and actin, and the force generati...

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Problems solved by technology

[0004] However, the existing experimental platforms all have the following disadvantages: i) the integration of the system is not high, and the disassembly and assembly are complicated, which is not conducive to the cleaning and maintenance of the experimental tank; ii) the device for detecting the length of the sarcomere is very complicated (if the optical path Diffraction mode is more complex), demanding on the environment and low stability; iii) more importantly, these platforms are developed for the mechanical properties of muscle fibers, and the stimulation of muscle fibers is in the form of simple pulse potentials, which cannot be applied The research on the principle of muscle fiber operation regulation is also incapable of real-time control of muscle fiber contraction; iv) the existing platforms have only one degree of freedom, and it is impossible to conduct experimental research on the complex control of muscle fibers

Recently, a new skeletal muscle biomechanical model——4M model has been proposed. This model has completed the unification of muscle microscopic and macroscopic mechanical properties through a bottom-up method, but there is still a lack of related experimental platforms.

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