Monoclonal antibody hybrid tumor cell strain KGH-R1 of broad spectrum anti-human p21Ras protein and monoclonal antibody

Abstract

The invention discloses a monoclonal antibody hybrid tumor cell strain KGH-R1 of broad spectrum anti-human p21Ras protein and a monoclonal antibody, belongs to the field of medical biology, and in particular relates to the monoclonal antibody hybrid tumor cell strain KGH-R1 of broad spectrum anti-human p21Ras protein and the monoclonal antibody. The hybrid tumor is preserved in a China center for type culture collection in September 23rd 2011, and has the preservation number of CCTCCNO: C201197. A mouse monoclonal antibody which has synchronous antagonism to three types of active Ras protein is finally obtained; and the monoclonal antibody which aims at a p21Ras protein target can be used for protein clinical test, can also construct an intracellular antibody, has an active tumor prevention function by inhibiting tissue vicious transformation through resisting overexpressed Ras protein, and has an active target antitumous effect on the tumor which corresponds to the overexpressed Ras protein.

Description

technical field [0001] The invention belongs to the field of medical biology, and in particular relates to a broad-spectrum anti-human Ras protein monoclonal antibody hybridoma cell line KGH-R1 and the monoclonal antibody. Background technique [0002] Ras gene is an important cell proto-oncogene, and its name comes from the English rat sarcoma of rat sarcoma. The Ras gene family includes three main members, namely H-Ras, K-Ras and N-Ras, which are located on chromosomes 12, 11 and 1, respectively, and encode a protein with a molecular weight of about 21KD consisting of 188-189 amino acids. That is, the p21Ras protein, the amino acid sequences of the three p21Ras proteins have about 85% homology. As an extremely important signal transduction protein, p21Ras protein regulates the normal differentiation and proliferation of cells. [0003] The p21Ras protein localizes on the inner side of the cell membrane to exert its biological function. p21Ras protein has its own GTP hyd...

AI Technical Summary

Problems solved by technology

[0005] At present, there is no domestically produced Ras protein antibody in China, and most of the foreign commercialized antibodies are prepared by synthetic peptides targeting the common region of the three Ras proteins as immunogens. The antibodies screened in this way are directed against the peptide sequence, and the price of foreign antibodies is usually More expensive, which also limits the application of this protein in clinical testing

In addition, the protein exerts its biological function through folding to form a certain spatial conformation, then the antibody produced against the polypeptide sequence will occur. Due to the steric hindrance formed by the Ras protein during the folding process, the binding ability of the antibody to the antigenic determinant will be reduced. Decrease; the antigenic determinant antagonized by the antibody is folded into the interior of the protein during protein folding and cannot be exposed on the surface of the protein, so it cannot bind to the active Ras protein

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