Amplification primer of corbiculidae shellfish mitochondria COI (Cytochrome Oxidase I) gene
A technique for amplifying primers and mitochondria, applied in the direction of DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of large inter-species variability, obstacles to the use of molecular techniques, and low amplification efficiency of COI non-specific amplification primers
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[0007] The screening method of the amplification primer of the clam family mitochondrial COI gene of the present invention adopts three steps: a, utilize COI universal amplification primer (LCO1490F and HCO2198R, Folmer 1994) to amplify the COI sequence of part clam family species; b, from Among the part of the Clamidae COI sequences obtained above, the conserved sequence fragments were found by comparison, and multiple sets of amplification primer sequences were synthesized; c, the effect of amplifying the Clamidae mitochondrial COI gene was screened from the multiple sets of synthetic amplification primer sequences The best pair of primers.
[0008] Below in conjunction with example the present invention will be further described:
[0009] 1. Sample collection: From 2002 to 2011, a total of 126 individuals of 3 genera and 4 species of Clamidae were collected from the north and south coasts of China.
[0010] 2. DNA extraction: DNA extraction was performed on the collected s...
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