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Freezing liquid for preserving embryo, preparation method and application thereof

A technology of freezing liquid and embryos, which is applied in application, preservation of human or animal bodies, animal husbandry, etc., can solve the problem of low development rate of frozen sampling embryos, achieve good freezing effect, high concentration, and prevent the formation of large ice crystals Effect

Inactive Publication Date: 2014-04-23
石家庄天泉良种奶牛有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The freezing liquid of the present invention solves the problem of low developmental rate of embryos in freezing sampling by conventional ethylene glycol freezing liquid by adding specific concentration of high molecular weight non-permeable antifreeze protective agent arabinogalactan to conventional embryo freezing liquid

Method used

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  • Freezing liquid for preserving embryo, preparation method and application thereof
  • Freezing liquid for preserving embryo, preparation method and application thereof
  • Freezing liquid for preserving embryo, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation of embodiment 1.0.03EAS freezing liquid:

[0048] Put 0.03g of arabinogalactan in a 10ml volumetric flask, add PBS to near the mark, and let it stand at 4°C to dissolve; after it is completely dissolved, add 0.3424g of sucrose, and let it stand at 4°C to dissolve; after it is completely dissolved, add 0.021 gBSA (do not shake, prevent foaming), stand at 4°C to dissolve; after it is completely dissolved, add PBS to the mark; after mixing the liquid in the volumetric flask, take 9.2ml of the solution in the volumetric flask and 0.8ml of pure liquid Ethylene glycol was mixed, sealed, and left to stand in the refrigerator at 4°C until use.

Embodiment 2

[0049] The preparation of embodiment 2.0.005EAS freezing liquid:

[0050] The preparation steps are the same as in Example 1, adding 0.005 g of arabinogalactan, and the amounts of sucrose, pure ethylene glycol and bovine serum albumin are the same as above.

Embodiment 3

[0051] The preparation of embodiment 3.0.01EAS freezing liquid:

[0052] The preparation steps are the same as in Example 1, adding 0.01 g of arabinogalactan, and the amounts of sucrose, pure ethylene glycol and bovine serum albumin are the same as above.

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Abstract

The invention discloses a freezing liquid for preserving embryo, especially a freezing liquid for preserving sampled embryo. The freezing liquid comprises ethylene glycol and sucrose, and is characterized by comprising 0.4-5g of arabinogalactan, 75-85mL of ethylene glycol, 30-40g of sucrose, and 910-930mL of PBS buffer solution containing 0.0021 g / mL bovine serum albumin (BSA). A preparation method of the freezing liquid comprises the steps of dissolving ethylene glycol, sucrose and arabinogalactan in the BSA-containing PBS buffer solution. The freezing liquid fully utilizes synergic effects of the four components that ethylene glycol, sucrose, arabinogalactan and bovine serum albumin are coordinated and supported one another, and has higher development rate of thawed sampled embryo in comparison with conventional ethylene glycol freezing liquid.

Description

technical field [0001] The invention relates to a freezing liquid for preserving embryos, in particular to a freezing liquid for preserving sampled embryos. The invention also relates to the preparation method and application of the freezing liquid. Background technique [0002] Embryo freezing is a method of embryo preservation. Embryos are placed in freezing liquid for protection and special cooling procedures are adopted to stop the metabolism of embryos at ultra-low temperatures (liquid nitrogen -196°C, liquid helium -269°C). When the embryos need to be used, they can be restored to viability through specific thawing procedures, so as to achieve the purpose of long-term preservation of embryos. [0003] For gender identification and other genetic testing and analysis, it is often necessary to use micromanipulation techniques to cut off a small part of embryonic tissue for analysis, and freeze the remaining part for future use. [0004] However, after the embryo is cut ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 余文莉李树静
Owner 石家庄天泉良种奶牛有限公司
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