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Gene group test structure

A detection structure and gene detection chip technology, applied in biochemical instruments, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problem that diagnostic chips are not easy to popularize in clinical medical applications, detection specificity is difficult to improve, and detection costs advanced questions

Inactive Publication Date: 2012-11-07
FOOYIN UNIV HOSPITAL
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Problems solved by technology

[0004] Although there have been many reports on the application of nylon membrane chips in molecular diagnosis and drug efficacy evaluation, due to the interpretation method used by the original nylon membrane chips, under the concept that the importance of each gene in a specific disease is equal, As a result, the detection specificity is not easy to improve after reaching a certain level
In addition, due to the high cost of the digitalis (Digoxigenin) system required for the Colorimetric Biochip operating platform, the cost of detection is too high. Assessing mature diagnostic chips is still not easy to popularize in clinical medicine applications
Therefore, it generally cannot meet the needs of users in actual use.

Method used

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Embodiment Construction

[0031] see figure 1 and figure 2 Shown are a schematic diagram of the gene group detection structure of the present invention and a schematic diagram of gene position arrangement of the gene detection chip of the present invention, respectively. As shown in the figure: the present invention is a gene group detection structure (WEnCA-Chipball) 100, which at least includes a gene detection chip 10, a sample pre-processing unit 20, a hybrid reaction area 30, a chip color rendering unit 40 and an analysis and interpretation unit 50 constitute.

[0032] The above-mentioned gene detection chip 10 is coated with a specific sequence, and is a screening chip with complete disease diagnosis, drug efficacy evaluation or genetic disease-causing genes.

[0033] The sample pretreatment unit 20 adds the sample to the lysis buffer to break the cells, extracts the mRNA, and binds the RNA in the cell by adding magnetic particles (Magnetic Particles) containing Poly-T primers, and lyses the n...

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Abstract

A gene group test structure at least comprises a gene test chip, a specimen pretreatment unit, a hybridization area, a chip color development unit and an analyzing and identifying unit. In this way, a WEnCA-Chipball operating platform, which is high in speed, accuracy and sensitivity and low in cost, allows quantitative analysis and is easy to combine with automation, is provided to conduct rapid test and analysis of a biological specimen, achieving the aim which cannot be realized by conventional biomedical test.

Description

technical field [0001] The present invention relates to a gene group detection structure, especially relates to an improvement of the shortcomings of the original nylon diaphragm operating platform, especially refers to the establishment of a new low-cost WEnCA (Weighted Enzymatic Chip Array) operating platform, which can be assisted by automatic operation to enable detection The time is greatly reduced, and human error is reduced, so as to break through the bottleneck faced by chip detection technology in the commercialization process. Background technique [0002] Analysis of gene overexpression has led to fundamental and clinical advances in disease diagnosis. The techniques for studying gene transition expression include Northern Blot, Reverse Transcription Polymerase Chain Reaction (RT-PCR) and real-time quantitative polymerase chain reaction (Real-Time PCR). ). Among them, Northern Blot is limited to research operations and cannot be practically applied to clinical ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
Inventor 林绣茹张惠人曹德安
Owner FOOYIN UNIV HOSPITAL
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