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Manufacture method of silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and application thereof

An immunosensor, iron tetroxide technology, applied in the field of new nano functional materials and biosensors, can solve the problems of expensive equipment, complex equipment, long analysis time, etc., achieve high sensitivity and rapid detection, simple preparation process, The effect of increasing sensitivity

Active Publication Date: 2013-10-09
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006]1. High-performance liquid chromatography: including liquid-liquid partition chromatography, liquid-solid chromatography, and ion-exchange chromatography. High, high sensitivity, but the price of the liquid chromatograph and the daily maintenance cost are expensive, and the analysis time is long
[0007]2. Liquid chromatography-tandem mass spectrometry: low detection limit, accurate qualitative and quantitative, and good reproducibility, but the equipment is complex and the operation process is cumbersome. The operation skills required are very high, and it is not suitable for on-site detection and rapid detection
[0008]3. Microbiological method: complex procedures, slow analysis speed and large error in results
[0017] CN200610130646.7 and CN200810052119.8 disclose two methods for detecting antibiotics based on surface plasmon resonance, which have the advantages of short measurement time, high precision and good specificity and other advantages, but the equipment is expensive

Method used

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  • Manufacture method of silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and application thereof
  • Manufacture method of silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and application thereof
  • Manufacture method of silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The preparation method of the silver hybrid mesoporous Fe3O4 antibiotic immunosensor comprises the following steps.

[0064] (1) Preparation of thionine-graphene mixed solution, the steps are as follows:

[0065] 1) Preparation of chitosan solution: Add 0.15 g of chitosan to 50 mL of acetic acid with a volume fraction of 1% and stir for 2 h to prepare a chitosan solution with a mass fraction of 0.3%;

[0066] 2) Preparation of thionine-chitosan solution (solution Ⅰ): 12 mg thionine was added to 4 mL chitosan solution with a mass fraction of 0.3%, ultrasonicated for 30 min to obtain 3 mg·mL -1 solution I;

[0067] 3) Preparation of graphene-chitosan solution (solution Ⅱ): 12 mg graphene was added to 4 mL chitosan solution with a mass fraction of 0.3%, and 3 mg·mL was obtained by ultrasonication for 30 min -1 solution II;

[0068] 4) Preparation of thionine-graphene mixed solution (solution Ⅲ): mix solution Ⅰ and solution Ⅱ at a volume ratio of 2 : 1 Mix and sonicat...

Embodiment 2

[0082] The preparation method of the silver hybrid mesoporous Fe3O4 antibiotic immunosensor comprises the following steps.

[0083] (1) Preparation of thionine-graphene mixed solution, the steps are as follows:

[0084] 1) Preparation of chitosan solution: add 0.25 g chitosan to 50 mL of acetic acid with a volume fraction of 1% and stir for 2 h to prepare a chitosan solution with a mass fraction of 0.5%;

[0085] 2) Preparation of thionine-chitosan solution (solution Ⅰ): 8 mg thionine was added to 2 mL chitosan solution with a mass fraction of 0.5%, and 4 mg·mL was obtained by ultrasonication for 30 min -1 solution I;

[0086]3) Preparation of graphene-chitosan solution (solution Ⅱ): 8 mg graphene was added to 2 mL chitosan solution with a mass fraction of 0.5%, and 4 mg·mL was obtained by ultrasonication for 30 min -1 solution II;

[0087] 4) Preparation of thionine-graphene mixed solution (solution III): mix solution I and solution II in a volume ratio of 3 : 1 Mix an...

Embodiment 3

[0101] The preparation method of the silver hybrid mesoporous Fe3O4 antibiotic immunosensor comprises the following steps.

[0102] (1) Preparation of thionine-graphene mixed solution, the steps are as follows:

[0103] 1) Preparation of chitosan solution: Add 0.35 g of chitosan to 50 mL of acetic acid with a volume fraction of 1% and stir for 2 h to prepare a chitosan solution with a mass fraction of 0.7%;

[0104] 2) Preparation of thionine-chitosan solution (solution Ⅰ): 15 mg thionine was added to 3 mL chitosan solution with a mass fraction of 0.7%, and 5 mg·mL was obtained by ultrasonication for 30 min -1 solution I;

[0105] 3) Preparation of graphene-chitosan solution (solution Ⅱ): 15 mg graphene was added to 3 mL chitosan solution with a mass fraction of 0.7%, and 5 mg·mL was obtained by ultrasonication for 30 min -1 solution II;

[0106] 4) Preparation of thionine-graphene mixed solution (solution III): mix solution I and solution II in a volume ratio of 5 : 1 ...

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Abstract

The invention discloses a manufacture method of a silver hybridization mesoporous ferroferric oxide antibiotic immunosensor and the application thereof. The manufacture method of the electrochemical immunosensor includes modifying thionine-graphene mixed solution on the surface of a glassy carbon electrode, conducting cross linking on an antibiotic antibody incubated by Ag-Fe3O4 mesoporous nanometer particles, closing non-specificity active sites by bovine serum albumin to manufacure the antibiotic electrochemical immunosensor. A detection method of antibiotic is that a reference electrode-saturated calomel electrode, an electrode-platinum filament electrode and a working electrode are correctly connected on an electrochemical working station, and immunodetection is conducted through the square wave voltammetry. The antibiotic electrochemical immunosensor has high sensitivity and selectivity, is simple in detection method and has the advantages of being quick, high in efficiency, good in specificity, low in cost, convenient to operate and the like. It takes to 2-3 minutes to finish one detection process.

Description

technical field [0001] The invention relates to a preparation method and application of a silver hybrid mesoporous iron ferric oxide antibiotic immunosensor. Specifically based on Ag-Fe 3 o 4 A label-free electrochemical immunosensor for detecting various antibiotics constructed by a nanocomposite material belongs to the technical field of new nanometer functional materials and biosensors. Background technique [0002] Antibiotics are drugs against pathogenic microorganisms. They are a class of secondary metabolites produced by microorganisms (including bacteria, fungi, and actinomycetes) or higher animals and plants during their life. Other chemical substances that function in the development of living cells. [0003] Commonly used antibiotics include extracts from microbial culture fluids and chemically synthesized or semi-synthesized compounds, which are divided into the following categories: β -Lactams, aminoglycosides, tetracyclines, chloramphenicols, macrolides, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N27/327
Inventor 魏琴杜斌于淑君张勇于海琴闫良国李贺王志玲吴丹罗川南马洪敏李玉阳朱宝存孙蒙曹伟
Owner UNIV OF JINAN
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