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Method for selecting goose carcass trait microsatellite molecular marker

A technology of carcass traits and molecular markers, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of low selection accuracy, incomplete matching, and short breeding cycle, so as to improve selection intensity, Accelerated breeding process, high precision effect

Inactive Publication Date: 2012-10-10
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for selecting meat goose carcass traits, which is easily affected by various factors, and the phenotype and its genotype are not completely consistent, so that the selection accuracy is low and the cycle is long. Molecular genetic marker selection method for meat goose carcass traits selection based on the genotype of microsatellite markers of meat goose, which can start early in life, has high accuracy, short breeding cycle and does not require slaughter tests

Method used

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Examples

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Effect test

Embodiment 1

[0029] Embodiment 1: (selection of Taihu goose carcass traits)

[0030] (1) Design specific primers for goose RY36 site: design a pair of specific primers RYF: 5’-TCCCAGCTTCACTCCTTTTC-3’ and RYR: 5’- GTGGTGTTCTTGCGGTGTAG-3’ according to the flanking sequences at both ends of goose microsatellite site RY36;

[0031] (2) Determination of carcass traits of Taihu geese: The indicators for the determination of carcass traits of Taihu geese include slaughter rate, half evisceration rate, full evisceration rate, chest muscle rate and leg muscle rate. Manual" operation;

[0032] (3) PCR amplification reaction: 150 individuals of the reserved species were selected from the Taihu goose population, blood was collected from wing veins, blood genomic DNA was extracted according to the conventional phenol-chloroform method, and PCR amplification reaction was carried out using the above primers and genomic DNA templates. The reaction system used was 10× Ex Taq Buffer (Mg 2+ Plus) 2.5 μL...

Embodiment 2

[0038] Embodiment 2: (selection of Lande goose carcass traits)

[0039] (1) Design specific primers for goose RY36 site: design a pair of specific primers RYF: 5'-TCCCAGCTTCACTCCTTTTC-3' and RYR: 5'- GTGGTGTTCTTGCGGTGTAG-3' according to the flanking sequences at both ends of goose microsatellite site RY36;

[0040] (2) Determination of Lande goose carcass proterties: the index of determination has slaughter rate, half evisceration rate, full evisceration rate, breast muscle rate and leg muscle rate, with reference to Chen Weisheng, 2006 " livestock and poultry genetic resources investigation technical manual " operation;

[0041] (3) PCR amplification reaction: Select 100 individuals of the reserved species in the Lande goose population, collect blood from the wing vein, extract blood genomic DNA by conventional phenol-chloroform method, and use the above primers and genomic DNA template for PCR amplification reaction. The reaction system used was 10× Ex Taq Buffer (Mg 2...

Embodiment 3

[0047] Embodiment 3: (selection of Zhejiang white goose carcass traits)

[0048] (1) Design specific primers for goose RY36 site: design a pair of specific primers RYF: 5'-TCCCAGCTTCACTCCTTTTC-3' and RYR: 5'- GTGGTGTTCTTGCGGTGTAG-3' according to the flanking sequences at both ends of goose microsatellite site RY36;

[0049] (2) Determination of carcass traits of Zhejiang white goose

[0050] The indicators for the determination of carcass traits of Zhedong White Goose include: slaughter rate, half-eviscerated rate, fully eviscerated rate, breast muscle rate and leg muscle rate, refer to the "Technical Manual for Investigation of Livestock and Poultry Genetic Resources" (Chen Weisheng, 2006);

[0051] (3) PCR amplification reaction: Select 200 individuals of the reserved species in the East Zhejiang white goose population, collect blood from wing veins, extract blood genomic DNA by conventional phenol-chloroform method, and use the above primers and genomic DNA templates for...

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Abstract

The invention discloses a method for selecting goose carcass trait microsatellite molecular marker and belongs to the technical field of poultry breeding. The method comprises the processes of: (1) designing specific primers of goose RY 36-site, wherein an upstream primer is 5'-TCCCAGCTTCACTCCTTTTC-3', and a downstream primer is 5'-GTGGTGTTCTTGCGGTGTAG-3'; (2) measuring goose carcass trait; (3) carrying out a PCR (polymerase chain reaction) amplification reaction; (4) carrying out polymorphism analysis of a PCR amplification product, and (5) selecting a superior goose parent individual body. According to the invention, goose carcass trait selection is carried out according to the genotype, the accuracy is high and the operation is simple. The carcass trait selection is begun at an earlier period of goose life; a generation interval is shortened; the breeding process of the goose is accelerated; the selection time can be shortened by about three months in each generation, and the method can be generalized and applied in the field of poultry breeding.

Description

technical field [0001] The invention relates to the technical field of poultry selection, in particular to a method for selecting microsatellite molecular markers for meat goose carcass traits. Background technique [0002] The carcass traits of meat goose mainly include slaughter rate, half evisceration rate, full evisceration rate, abdominal fat rate, breast muscle rate and leg muscle rate, which are important economic traits in the production of meat geese, and are increasingly valued in the breeding of meat geese . Since carcass traits are economic traits that cannot be measured by living bodies, the traditional selection methods for meat goose carcass traits are: (1) selection based on pedigree data, that is, selection of good bloodlines for breeding, and elimination of poor bloodlines; (2) ) Selection based on sibling performance, that is, to estimate the carcass traits of breeding poultry based on the carcass performance of full siblings or half siblings; (3) Selecti...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 李国勤卢立志李进军沈军达陶争荣王德前田勇陈黎徐坚
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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