Non-tissue-cultured walnut micro-cutting grafting seedling raising method

Abstract

The invention discloses a non-tissue-cultured walnut micro-cutting grafting seedling raising method. A walnut grafted seedling which is cultured by using soil is used as a female parent tree for producing micro-cuttings, the nutrition of the tree is increased by flexibly adopting a rhizosphere fertilization mode and a leaf spray fertilization mode according to the growth vigor of a nursery stock, the germination speed is high, and the production period of the micro-cuttings is short; the female parent tree is planted in a plastic greenhouse and an open field respectively, so that complementation in time can be realized, the production time of the micro-cuttings is prolonged, and the cost is reduced; the walnut grafted seedling which is cultured by using soil is used as the female parent tree for producing the micro-cuttings, the produced micro-cuttings has the base diameter of greater than 0.4 cm, is well matched with seedlings in the roughness, and is robust in the growth, and thus the problems of leaf yellowing, withering, slimness and weakness and the like of the tissue-cultured micro-cuttings which occur frequently are solved; the method is easy to operate without specialized training, and the grafting survival rate of normal personnel reaches 79 percent; the requirements on environment conditions are loose, i.e., the environment conditions can be spring, summer and autumn, the micro-cuttings can be produced in the outdoor fields under natural conditions, and the propagation coefficient is large; and by applying ethephon, the growth speed of the micro-cuttings is flexibly controlled, and adjustment and control are performed to enable that the production of the micro-cuttings is consistent with that of the seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant vegetative propagation, in particular to a non-tissue culture walnut micro branch grafting seedling raising method. Background technique [0002] Walnut is an important dried fruit tree species in my country, with wide distribution, many uses and high economic value. However, the seeds are propagated by seeds, resulting in mixed quality and low yield. Because walnuts are rich in tannins and have severe wounds, asexual reproduction (grafting, cuttings, tissue culture) is relatively difficult, thus restricting the process of improving walnut varieties in my country. For decades, people have devoted themselves to the exploration of walnut asexual propagation methods. In order to alleviate the impact of tannin and wound flow on the survival rate of grafting, indoor grafting has been developed (in the northern region, the annual rootstocks are excavated from the field after falling leaves and buried und...

AI Technical Summary

Problems solved by technology

[0011] The present invention replaces tissue-cultured micro-branches with micro-branches produced by a specific technical method by conventionally grafting seedlings of excellent varieties of walnut planted in the ground, and mainly solves the problem of large proportion of micro-branches in walnut tissue culture and low utilization rate, and avoids tissue culture of walnut micro-branches. A series of problems such as large investment in production equipment and facilities, strong technical aseptic operation required in the whole process, cumbersome operation, and high production cost, in order to achieve rapid reproduction and increase the reproduction coefficient

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