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Enterobacter agglomerans with phosphate-solubilizing capacity, and application thereof

A technology of Enterobacter agglomerans and Enterobacter agglomerates, which is applied in the fields of application, bacteria, seeds and rhizome treatment, etc., can solve the problems of unsatisfactory pollution remediation, weak growth mitigation phosphorus ability, etc., and achieve environmental friendly cost, promote growth, high The effect of phosphorus solubilizing ability

Inactive Publication Date: 2014-01-01
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, wild phosphate-solubilizing bacteria in nature have a weak ability to grow and relieve phosphorus, and in polluted environments, the growth of wild strains is inhibited, which cannot meet the needs of pollution remediation.

Method used

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  • Enterobacter agglomerans with phosphate-solubilizing capacity, and application thereof
  • Enterobacter agglomerans with phosphate-solubilizing capacity, and application thereof
  • Enterobacter agglomerans with phosphate-solubilizing capacity, and application thereof

Examples

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Effect test

Embodiment 1

[0055] Example 1: According to the method for mutagenesis and breeding of Enterobacter agglomerans with phosphorus-solubilizing ability provided by the present invention, mutagenesis screening can tolerate the strong mutagenized strain of heavy metal lead phosphorus-solubilizing ability, comprising the following steps:

[0056] 1) Enterobacter agglomerans KMC with high phosphorus-solubilizing ability screened from the plant rhizosphere in the laboratory was used as the starting strain;

[0057] 2) Mutation breeding

[0058] (1) Prepare the single-cell suspension of the starting strain KMC

[0059] The starting strain KMC was inoculated in liquid medium A, cultured at 28°C, 150rpm for 12hrs, centrifuged, washed with sterile physiological saline, placed in a Erlenmeyer flask equipped with glass beads, and oscillated to disperse into a single-cell bacterial suspension liquid;

[0060] The composition of the medium A is: tryptone 5 g, yeast powder 5 g, NaCl 10 g, distilled wat...

Embodiment 2

[0070] Example 2: RKMC-7 on Pb 2+ resistance, the determination steps are as follows:

[0071] Inoculate RKMC-7 in liquid medium A for enrichment culture, grow to exponential phase, centrifuge at 8000 rpm for 10 min, wash with 0.85% sterile NaCl solution three times, then adjust the bacterial solution with 0.85% sterile NaCl solution Concentration is 10 7 CFU / ml, draw 1 mL and add 80 mL of Pb with different concentrations 2+ ( 0, 400, 800, 1000, 1200mg Pb 2+ / l ) in a 250 mL Erlenmeyer flask of liquid medium D, Pb2+ With Pb(NO 3 ) 2 After adding in the form of 28 ° C for 5 days, the absorbance value of the culture solution was measured at 600 nm. The results showed that: in Pb 2+ When the concentration reaches 1000mg / L, RKMC-7 can still grow, indicating that RKMC-7 can tolerate higher concentrations of Pb 2+ , can grow in heavily polluted Pb-contaminated soils, and has the potential to remediate Pb-contaminated soils. RKMC-7 anti-Pb 2+ The growth chart is attached...

Embodiment 3

[0072] Example 3: RKMC-7 stability determination, the specific steps are as follows:

[0073] Quickly thaw the frozen RKMC-7 at 37°C, put 1% of the inoculum into a test tube containing 10ml of liquid medium A, culture at 28°C for 12hrs, centrifuge, and use 0.85% sterile After washing with NaCl solution for three times, suspend with 0.85% sterile NaCl solution, and adjust the cell concentration to 1×10 7 CFU / ml, insert in liquid medium B according to the inoculum size of 2%, cultivate 7 days at 150rpm, measure the content of soluble phosphorus in the supernatant with molybdenum blue colorimetry; 1% of the inoculum was then transferred to fresh liquid medium A, and the above steps were repeated for a total of 14 passages to determine the phosphorus-dissolving ability of RKMC-7. The results are shown in the attached Figure 6 , it can be seen from the figure that after 14 passages, the phosphorus-dissolving ability of RKMC-7 remained basically stable, which indicated that the ...

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Abstract

The invention discloses a strain of phosphate-solubilizing bacteria which is preserved in China general microbiological culture collection center (CGMCC) and is named as enterobacter agglomerans RKMC-7 in a classification way with the preservation number of CGMCC No. 5005. After a bactericide containing the enterobacter agglomerans is inoculated into the soil polluted by lead, the exchangeable form lead can be promoted to be fixed, and the fixing efficiency is 77-91.4%. The bactericide is simple in preparation method, rich in material source and easy in implementation of large-scale production, can realize the effect of carrying out agricultural production while repairing, and has important significance for guaranteeing grain safety and environmental protection.

Description

technical field [0001] The invention relates to an enterobacter agglomerans with phosphorus-solubilizing ability, and belongs to the technical field of microorganism application. Background technique [0002] With the rapid growth of global population, rapid industrialization and urbanization, heavy metal pollution has become a serious problem of soil pollution in China and the world. At present, the average annual emission of lead in the world is about 5 million tons. In the past 50 years, about 7.83×10 lead was released into the global environment 5 t. Most of them enter the soil, resulting in varying degrees of lead pollution in the soil of countries all over the world. According to data provided by the State Environmental Protection Administration of my country in 2003, nearly 1 / 5 of the arable land area in my country was polluted by lead, and the lead-exceeding rates in vegetables, grain, fruit, meat and animal products in the suburbs of large and medium-sized citie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C21/00A01C1/00A01G1/00B09C1/10C12N1/20C12R1/01
Inventor 朱晓丽范代娣马沛任永霞梁丽华申烨华
Owner NORTHWEST UNIV
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