Newcastle disease and avian infectious bronchitis bigeminal live vaccine heat-resisting freeze-drying protective agent and preparation method
A technology for chicken contagiousness and bronchitis, which is used in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of long-term storage and transportation, etc., and achieves high aging resistance and low virus loss rate. Effect
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Embodiment 1
[0019] Preparation of heat-resistant and freeze-dried vaccine of chicken Newcastle disease and chicken infectious bronchitis dual live vaccine
[0020] 1) Mix sodium carboxymethylcellulose with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare a concentration of 3% (the concentration involved in the present invention is all unless otherwise specified. is the mass percent concentration) of liquid A.
[0021] 2) Mix xylo-oligosaccharide, sorbitol and water for injection to prepare liquid B, the concentration of xylo-oligosaccharide is 8%, and the concentration of sorbitol is 3%.
[0022] 3) Mix sodium glutamate, sodium selenite, and water for injection, stir and mix well, and prepare liquid C with a concentration of sodium glutamate of 1.5% and a concentration of sodium selenite of 1.5%.
[0023] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.
[0024] 5) Mix t...
Embodiment 2
[0028] Preparation of heat-resistant and freeze-dried vaccine of chicken Newcastle disease and chicken infectious bronchitis dual live vaccine
[0029] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 3%.
[0030] 2) Mix xylo-oligosaccharides, sorbitol and water for injection to prepare liquid B, the concentration of xylo-oligosaccharides is 6%, and the concentration of sorbitol is 3%.
[0031] 3) Mix sodium glutamate, sodium selenite, and water for injection, stir and mix well, and prepare liquid C with a concentration of sodium glutamate of 1.5% and a concentration of sodium selenite of 0.3%.
[0032] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.
[0033] 5) Mix the lyoprotectant of the vaccine with the virus solution of the Newcastle disease and infectious bronchitis dual live vac...
Embodiment 3
[0037] Preparation of heat-resistant and freeze-dried vaccine of chicken Newcastle disease and chicken infectious bronchitis dual live vaccine
[0038] 1) Mix carboxymethylcellulose sodium with water for injection, heat at 98-100°C for 10-20 minutes, cool to room temperature, and prepare liquid A with a concentration of 6%.
[0039] 2) Mix xylo-oligosaccharides, sorbitol and water for injection to prepare liquid B, the concentration of xylo-oligosaccharides is 10%, and the concentration of sorbitol is 6%.
[0040] 3) Mix sodium glutamate, sodium selenite, and water for injection, stir and mix well, and prepare liquid C with a concentration of sodium glutamate of 1.5% and a concentration of sodium selenite of 0.3%.
[0041] 4) Prepare liquid A, liquid B, and liquid C at a volume ratio of 1:1:1 to make a vaccine freeze-drying protective agent.
[0042] 5) Mix the vaccine lyoprotectant with the virus solution of the Newcastle disease and infectious bronchitis dual live vaccine a...
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