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Dandelion polysaccharide extract and preparation method and application thereof

A polysaccharide extraction and dandelion technology, which is applied in the field of dandelion polysaccharide extract and its preparation, can solve the problems of no anti-complement activity of dandelion and its polysaccharide extract, and achieve broad application prospects and market value, significant anti-complement activity, and preparation simple craftsmanship

Inactive Publication Date: 2014-03-05
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no relevant research report on the anti-complement activity of dandelion and its polysaccharide extracts

Method used

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  • Dandelion polysaccharide extract and preparation method and application thereof
  • Dandelion polysaccharide extract and preparation method and application thereof
  • Dandelion polysaccharide extract and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030]Take the whole dandelion herb and place it in the extractor, add ethanol with a volume percentage of 95%, the volume of ethanol added is twice the mass of the whole dandelion herb; carry out reflux defatting for 2 hours, filter, and evaporate the ethanol in the medicinal residue; Add deionized water to the medicinal residues, soak at room temperature for 3 hours, then heat and boil for 4 hours, filter, and repeat the above operation 3 times for the obtained medicinal residues; the volume of water added each time is 10 times the quality of the medicinal residues; combined Extract solution, concentrate the extract solution to a relative density of 1.2 at 80°C, centrifuge, and collect the supernatant; add ethanol with a volume percentage of 95% to the supernatant under constant stirring, and the volume of ethanol added is the supernatant 4 times the volume of the solution; after adding, put it in a refrigerator at 4°C for 12 hours; centrifuge to get the precipitate, and evap...

Embodiment 2

[0041] Take the whole dandelion herb and place it in the extractor, add ethanol with a volume percentage of 95%, and the volume of ethanol added is 3 times the mass of the whole dandelion herb; carry out reflux defatting for 2 hours, filter, and evaporate the ethanol in the medicinal residue; Add deionized water to the medicinal residues, soak at room temperature for 2 hours, then heat and boil for 3 hours, filter, and repeat the above operation 2 times for the obtained medicinal residues; the volume of water added each time is 9 times the quality of the medicinal residues; combine Extract solution, concentrate the extract solution to a relative density of 1.1 at 70°C, centrifuge, and collect the supernatant; add ethanol with a volume percentage of 95% to the supernatant under constant stirring, and the volume of ethanol added is the supernatant 3 times the volume of the solution; after adding, put it in a refrigerator at 4°C for 12 hours; centrifuge to obtain a precipitate, an...

Embodiment 3

[0044] Take the whole dandelion herb and place it in the extractor, add ethanol with a volume percentage of 95%, and the volume of ethanol added is 4 times the mass of the whole dandelion herb; carry out reflux defatting for 2 hours, filter, and evaporate the ethanol in the medicinal residue; Add deionized water to the medicinal residues, soak at room temperature for 1 hour, then heat and boil for 2 hours, filter, and repeat the above operation once for the obtained medicinal residues; the volume of water added is 8 times the mass of the medicinal residues; combine the extracts , concentrate the extract to a relative density of 1.5 at 60°C, centrifuge, and collect the supernatant; add ethanol with a volume percentage of 95% to the supernatant under constant stirring, and the volume of ethanol added is the volume of the supernatant After adding, put it in a refrigerator at 4°C for 12 hours; centrifuge to obtain a precipitate, and evaporate the ethanol; dissolve the precipitate i...

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Abstract

The invention discloses dandelion polysaccharide extract and a preparation method and application thereof. The preparation method of the dandelion polysaccharide extract includes subjecting a whole plant of dandelion to water extraction and ethanol precipitation, capturing components larger than 10000Da by dialysis, and subjecting the components to anion exchange column chromatographic separation to obtain the dandelion polysaccharide extract. The dandelion polysaccharide extract contains 7-8wt% of polysaccharide A, 22-23wt% of polysaccharide B, 17-18wt% of polysaccharide C, 6-7wt% of polysaccharide D, 1-2wt% of polysaccharide E, and 0.5-1.5wt% of polysaccharide F. The dandelion polysaccharide extract has evident anticomplementary activity, can be made into drugs and functional foods for preventing and / or treating systemic immunodeficiencies, and is safe to use and free of side effects. The preparation method of the dandelion polysaccharide extract is simple, the quality of the dandelion polysaccharide extract is stable, and the dandelion polysaccharide extract is applicable to large-scale production and has broad application prospect and market value.

Description

technical field [0001] The invention relates to a dandelion polysaccharide extract, a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. Background technique [0002] Dandelion (Herba Taraxaci) is a perennial herb of Compositae, also known as chrysanthemum chrysanthemum and chrysanthemum chrysanthemum. Have heat-clearing and toxic substances removing, reducing swelling and dissipating stagnation, inducing diuresis to relieve stranguria, and are used for boils and swollen toxins, mastitis, scrofula, conjunctival congestion, sore throat, lung abscess, intestinal fistula, damp-heat jaundice, hot stranguria and astringent pain. Modern pharmacological studies have shown that dandelion has the effects of promoting gallbladder and protecting liver, anti-endotoxin, anti-cancer, hypoglycemic, anti-coagulant, diuretic, invigorating spleen and stomach, prebiotics, broad-spectrum antibacterial and immune promotion. [0003] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C08L5/00A23L1/09A61K31/715A61P37/06
Inventor 王顺春刘飞王宏伟鲍斌范洪伟施松善王峥涛胡之璧
Owner SHANGHAI UNIV OF T C M
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