Nematicidal composition containing verticillium chlamydosporium and Paecilomyces lilacinus and preparation method thereof
The technology of Paecilomyces lilacinus and Verticillium pachysporum is applied in the field of compositions for killing nematodes by using nematode parasites and the field of preparation thereof, which can solve the problem of ineffectiveness of pesticide varieties, unavailability, long development cycle of new pesticides, etc. problems, to overcome the long development cycle of new drugs, promote growth, and have good nematicide effects.
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[0039] A preparation method of a nematicidal composition containing Verticillium pachyspora and Paecilomyces lilacinus combined, comprising the following steps:
[0040] (1) Disperse the mother powder of Verticillium pachyspora and Paecilomyces lilacinus uniformly in a homogenizer equipped with solvents and additives to obtain a mixture of Verticillium pachyspora and Paecilomyces lilacinus with uniform viscosity bacterial liquid;
[0041] (2) Transfer the prepared mixed bacterial liquid to a high-level container;
[0042] (3) Put the carrier into the mixer;
[0043] (4) Start the stirrer, open the discharge valve of the high-level container, and add the mixed bacterial liquid until the bacterial liquid is added;
[0044] (5) Check the effect of the adsorption method for granulation, and check the technical indicators of the product.
Embodiment 1
[0045] Example 1 Study on the synergistic effect
[0046] In order to overcome and delay the resistance of pests and prolong the service life of old pesticide varieties, we used Verticillium pachysporum and Paecilomyces lilacinus to combine pesticides to study the synergistic effect on R. incognita.
[0047] Pick tomato roots artificially inoculated with Nematode incognita, crush them with a tissue masher, pass through 200-mesh, 325-mesh, and 500-mesh nematode standard sieves in turn, rinse the eggs on the 500-mesh sieve with sterile water, and prepare eggs Suspension (100 eggs / mL). Another part of the eggs was incubated in sterile water, and the live larvae (2nd instar larvae) hatched on the second day were used for testing. The fresh Paecilomyces lilacinus fermentation broth was prepared into a mother liquor of a certain concentration, and then mixed with Verticillium pachysporum according to (6:1, 4:1, 2:1, 1:1 and 1:2) for the test. Nematode larvae or egg suspension (100...
Embodiment 2
[0048] Preparation of the composition of Example 2
[0049] The invention provides a case of a nematicidal granule containing a combination of Verticillium pachyspora and Paecilomyces lilace and a preparation method thereof:
[0050] The components and contents in the granules can be determined according to 1) 50 grams of live mother powder of Verticillium pachysporum 2.5 billion per gram, 50 grams of mother powder containing 20 billion per gram of Paecilomyces lilacinus, stabilizer soybean oil 50 grams grams, 100 grams of solvent mineral oil, 750 grams of carrier kaolin. 2) 50 grams of live mother powder containing 2.5 billion / gram Verticillium pachyspora, 50 grams of mother powder containing 20 billion / gram Paecilomyces lilacinus, 50 grams of methyl solvate, 50 grams of stabilizer castor oil, carrier Kaolin about 800 grams.
[0051] The Verticillium pachyspora and Paecilomyces lilacinacea nematicidal granules are prepared through the following steps: (1) The mother powder ...
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