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Antisense oligonucleotide of human mir-504 and its application

A technology of mir-504 and oligonucleotide, applied in the field of human microRNA-504 antisense oligonucleotide and its application, microRNAs antisense oligonucleotide, can solve the problem of poor curative effect and long-term The curative effect of patients with metastases is poor, and achieve the effect of inhibiting malignant proliferation, good inhibitory effect, and long half-life

Active Publication Date: 2015-07-29
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, these methods have their own limitations, especially for the middle-advanced and relapsed patients, and the curative effect is even worse for those with distant metastasis.

Method used

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  • Antisense oligonucleotide of human mir-504 and its application
  • Antisense oligonucleotide of human mir-504 and its application
  • Antisense oligonucleotide of human mir-504 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] Example: Detection of antisense oligonucleotides of miR-504 on human glioblastoma cell line U87 / MG inhibitory activity

[0030] First, miR-504 antisense oligonucleotide was synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd., the sequence is: 5'-GAUAGAGUGCAGACCAGGGUCU-3'. All the sequences used in the examples were synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0031] Cell culture:

[0032] U87 / MG cells (human brain astroglioblastoma, purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences) were purchased from Hyclone, FBS in 10% FBS-DMEM medium (DMEM (a conventional medium) (fetal bovine serum) purchased from Gibco), at 37 ° C, 5% CO 2 cultivated under conditions. Collect U87 / MG cells in good growth state, count by centrifugation, and use 2×10 3 Spread each well in a 96-well plate at 37°C, 5% CO 2 Cultivate for 24h.

[0033] Transfection:

[0034] 1) One day before transfection, ...

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Abstract

The invention discloses an antisense oligonucleotide for inhibiting expression of the human microRNA-504 and application thereof. The antisense oligonucleotide specifically binds to the human miR-504, and contains sequences which are complementary to 13-22 continuous nucleotides in the following nucleotide sequence 5'-AGACCCUGGUCUGCACUCUAUC-3', especially contains the sequence 5'-GAUAGAGUGCAGACCAGGGUCU-3'. The antisense oligonucleotide of the invention can be ribonucleotide, deoxyribonucleotide or chimera of ribonucleotide and deoxyribonucleotide, and any nucleotide in the chain can be modified. The miR-504 antisense oligonucleotide of the invention can effectively inhibit expression of miR-504 in the human glioma cell, and inhibit growth and propagation of the cell, thereby effectively treating glioma and other tumours with high expression of miR-504.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and the field of medicine. Specifically, the present invention relates to a microRNAs (miRNA) antisense oligonucleotide, in particular to human microRNA-504 (human miR-504) antisense oligonucleotide and applications thereof. The antisense oligonucleotide can be complementary to human miR-504, thereby inhibiting the expression of human miR-504 to play an anti-tumor effect. The present invention also relates to a pharmaceutical composition comprising the miRNA antisense oligonucleotide. Background technique [0002] miRNAs (also known as microRNA or microRNA) are small non-coding RNAs with a length of 20-25bp, usually transcribed by RNA polymerase II (Pol II), and generally the initial product is a large cap structure (7MGpppG) and pri-miRNA (primary miRNA, primary miRNA) of polyadenylic acid tail (AAAAA). These pri-miRNAs are processed into precursor miRNAs (precursor miRNA, pre-miR...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00A61P25/00
Inventor 丁侃张佩琢李捷沈孝坤
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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