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Antisense oligonucleotide of human mir-1274b and its application

A technology of oligonucleotides and ribonucleotides, applied in microRNAs antisense oligonucleotides, human microRNA-1274b antisense oligonucleotides and their applications, can solve the problem of improving the survival rate of tumor patients Obvious, poor curative effect in patients with distant metastasis, low survival rate in middle and advanced patients, etc., to achieve good inhibitory effect, ability to inhibit malignant proliferation, and long half-life

Active Publication Date: 2016-01-20
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the past 30 years, although the comprehensive treatment of tumors has been very common clinically, the comprehensive treatment based on surgery and supplemented by radiotherapy and chemotherapy has not significantly improved the survival rate of cancer patients, and the 5-year overall survival rate is still low, hovering At about 30% to 55%, there is no significant improvement, and the 5-year survival rate of middle and advanced patients is even lower, about 20%.
Moreover, these methods have their own limitations, especially for the middle-advanced and relapsed patients, and the curative effect is even worse for those with distant metastasis.

Method used

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  • Antisense oligonucleotide of human mir-1274b and its application
  • Antisense oligonucleotide of human mir-1274b and its application
  • Antisense oligonucleotide of human mir-1274b and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0030] Example: Detection of antisense oligonucleotides of human miR-1274b on human glioblastoma cell line U87 / MG inhibitory activity

[0031] First, miR-1274b antisense oligonucleotide was synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd., the sequence is: 5'-UGGCGCCCGAACAGGGA-3'. All the sequences used in the examples were synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0032] Cell culture:

[0033] U87 / MG cells (human brain astroglioblastoma, purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences), 10% FBS-DMEM medium (FBS (fetal bovine serum) were purchased from Gibco, DMEM (a A regular medium) purchased from Hyclone) and cultivated at 37°C, 5% CO 2 to cultivate. Collect U87 / MG cells in good growth state, count by centrifugation, and use 2×10 3 Spread each well in a 96-well plate at 37°C, 5% CO 2 Cultivate for 24h.

[0034] Transfection:

[0035] 1) One day before transfection, ino...

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Abstract

The invention discloses an antisense oligodeoxyncleotide for inhibiting the expression of human microRNA-1274b and the application thereof. The antisense oligodeoxyncleotide is specifically bound to human miR-1274b, and contains 13-17 continuous nucleotide complemented sequences among the following nucleotide sequence: 5'-UCCCUGUUCGGGCGCCA-3', particularly the sequence: 5'-UGGCGCCCGAACAGGGA-3'. The antisense oligodeoxyncleotide of the invention may be a ribotide, a deoxyribotide, or a chimera of a ribotide and a deoxyribotide, and can modify any nucleotide in a chain. The antisense oligodeoxyncleotide of miR-1274b of the invention can effectively inhibit miR1274b expression in human glioma cell, and can inhibit the growth and propagation of the human glioma cell, thereby effectively treating glioma and other tumours of miR-1274b high expression.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and the field of medicine. Specifically, the present invention relates to a microRNAs (miRNA) antisense oligonucleotide, in particular to human microRNA-1274b (human miR-1274b) antisense oligonucleotide and applications thereof. The antisense oligonucleotide can be complementary to human miR-1274b, thereby inhibiting the expression of human miR-1274b to play an anti-tumor effect. The present invention also relates to a pharmaceutical composition comprising the miRNA antisense oligonucleotide. Background technique [0002] miRNAs (also known as microRNA or microRNA) are small non-coding RNAs with a length of 20-25bp, usually transcribed by RNA polymerase II (PolII), and generally the initial product is a large cap structure (7MGpppG) and multiple pri-miRNA (primarymiRNA, primary miRNA) of polyadenylic acid tail (AAAAA). These pri-miRNAs are processed into 70 nucleotide precursor miR...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00A61P25/00
Inventor 丁侃张佩琢李捷沈孝坤
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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