Vaccine for preventing chicken diseases and preparation method thereof

A technology of vaccines and inactivated vaccines, applied in the field of poultry vaccines, can solve the problems of no particularly targeted preventive vaccines, and achieve the effects of normal egg production and eggshell quality, good protection effects, and simple methods

Inactive Publication Date: 2012-08-22
厦门生光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no vaccines specifically targeted to prevent diseases caused by infectious bronchitis and hepatitis bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: strain isolation method and standard

[0029] 1. Strain preparation

[0030] (1) Separation of strains:

[0031] Take a small piece of liver tissue (about 50 mg) from a sick chicken with infectious hepatitis (Fujian Xiangan Neicuo Chicken Farm) and put it in an EP tube filled with 300ul 1% TritonX-100, grind it with a grinding rod , take 100ul each to spread on LB plates containing ampicillin, cana and chloramphenicol respectively (ambenzyl: 0.05mg / ml; cana: 0.04mg / ml), and incubate at 37°C for 24h; There are colonies growing on the plate;

[0032] (2) Bacteria expansion culture

[0033] Pick a single colony from the above-mentioned long-growing plate into 250ml LB liquid medium containing both ampicillin and Cannabis (ampilin: 0.05mg / ml; Cannabis: 0.04mg / ml), and culture on a shaker at 37°C for 24-30 hours , measure OD 600 about 2.0;

[0034] (3) Identification of strains

[0035] Take 30ul of the above-mentioned expanded culture and send it to Sha...

Embodiment 2

[0048] Embodiment 2: prepare production bacterial classification

[0049] Dilute the Flavobacterium gallinarum strain obtained in Example 1 with sterilized physiological saline and inoculate it into susceptible chicks aged 2 to 7 days. Take a small piece (about 50 mg) of the liver tissue of the diseased chicken and put it in a container containing 300 ul of 1% TritonX-100 In the EP tube, grind it with a grinding rod to make a strain for production.

Embodiment 3

[0050] Embodiment 3: Vaccine manufacturing production process

[0051] 1. Concentration and inactivation time of formaldehyde inactivator

[0052] While ensuring the complete inactivation of bacteria, reduce the amount of formaldehyde used as much as possible, and inactivate the bacteria antigen solution at different final concentration of formaldehyde (0.1%, 0.2%, 0.3% and 0.4%) and inactivation time (3h, 6h, 12h, 24h) and 48h) multiple crossover experiments were performed. The research results show that: the final concentration of formaldehyde is 0.4%, the inactivation time is 48h, and the inactivation effect is the best;

[0053] 2. Production process of inactivated vaccines:

[0054] In order to improve the production process, after repeated comparisons of different production processes, the corresponding specifications have been established;

[0055] Antigen preparation uses Flavobacterium strains, inoculates susceptible chicken embryos aged 2 to 7 days to produce pat...

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PUM

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Abstract

The invention relates to a vaccine for preventing chicken diseases and a preparation method thereof, in particular to a chicken pathogenic flavobacterium strain with preservation code of CCTCCM2012100, and a vaccine containing chicken pathogenic flavobacterium. The preparation method of the vaccine includes diluting the chicken pathogenic flavobacterium strain in sterilized normal saline, and inoculating the strain to a 7-day-old susceptible chick by injecting; placing a piece of liver tissue of the diseased chick in an EP (epoxy) tube containing 1% TritonX-100, and grinding the piece of liver tissue with a grinding bar to make a production strain; emulsifying and packaging after inactivation. The vaccine can be used for preventing avian infectious laryngotracheitis, and avian diseases caused by hepatosis pathogen. The prepared inactivated vaccine has reliable quality, meets requirements, is high in safety, has good immune effect on chickens, is long in maintenance stage, has well protection effect on attach of strong bacteria, and has no influence on egg laying of chickens.

Description

technical field [0001] The invention relates to a poultry vaccine, in particular to a poultry disease vaccine and a preparation method thereof. Background technique [0002] For a long time, chickens are prone to outbreaks of diseases due to group living, especially infectious diseases, such as chicken infectious bronchitis and diseases caused by hepatitis bacteria. Common infectious diseases in chicken flocks have a great impact on breeding. If they are not prevented, they will bring huge economic losses to farmers. Therefore, preventive vaccines for infectious diseases have highlighted the importance. There is currently no vaccine specifically targeted to prevent diseases caused by infectious bronchitis and hepatitis bacteria. [0003] In addition, there are many ways to isolate bacteria, and the isolation methods are also different according to the different sources of bacteria and their own characteristics. Correct isolation and culture will help to quickly obtain susp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/20
Inventor 陈建明陈敏杨慧胡彬翁勤
Owner 厦门生光生物科技有限公司
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