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Preparation of silk-screen printing electrode for rapidly diagnosing diabetic ketoacidosis

A technology of screen printing electrodes and hydroxybutyric acid, which is applied in the direction of material analysis, measuring devices, instruments, etc. by electromagnetic means, can solve the problems of long time required, inability to detect samples in real time, complicated operation, etc.

Inactive Publication Date: 2012-08-15
湖南尔文水电建材有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method needs to formulate a standard curve in advance, so it takes a long time, the operation is complicated, and it cannot detect samples in real time

Method used

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  • Preparation of silk-screen printing electrode for rapidly diagnosing diabetic ketoacidosis
  • Preparation of silk-screen printing electrode for rapidly diagnosing diabetic ketoacidosis
  • Preparation of silk-screen printing electrode for rapidly diagnosing diabetic ketoacidosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Preparation of screen-printed electrodes for rapid diagnosis of diabetic ketoacidosis

[0017] Dissolve 1 mg of nitrocellulose, 2 mg of hydroxybutyrate dehydrogenase, 2 mg of potassium ferricyanide, 2 mg of reduced coenzyme and 1 mg of chitosan in 10 mL of phosphate buffer solution with a pH of 7.4. 1% glutaraldehyde solution was added to the above mixed solution and stirred for 0.5 hours, 10 uL solution was added dropwise on the working electrode 1, and dried at 45°C for 0.5 h; 1 mg nitrocellulose, 2 mg potassium ferricyanide, 2 mg of reduced coenzyme and 1 mg of chitosan were dissolved in 10 mL of phosphate buffer solution with a pH of 7.4, 5uL of 1% glutaraldehyde solution was added to the above mixed solution and stirred for 0.5 hours, and 10 uL of the solution was added dropwise to On the working electrode 2, dry at 45°C for 0.5 h to obtain a screen-printed electrode for rapid diagnosis of diabetic ketoacidosis.

Embodiment 2

[0018] Example 2 The current response of different concentrations of β-hydroxybutyric acid on the screen-printed electrode test paper

[0019] First, accurately weigh 0.198 g of β-hydroxybutyric acid and dissolve it in 10 mL of PBS solution (containing 0.1 mol L -1 KClO 4 ), the concentration is 100 mmol·L -1 , diluted with PBS solution, prepared 0.01, 0.03, 0.05, 0.06, 0.08, 0.1, 0.3, 0.5, 0.8, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0 ,8.0,9.0 and 10.0 mmol·L -1 The β-hydroxybutyric acid solution, using CHI660B electrochemical workstation to measure the current response of different concentrations of β-hydroxybutyric acid on the screen printing electrode test paper, the potential is 0.4 V, such as figure 2 shown. The concentration of β-hydroxybutyric acid is 0.05 – 5.0 mmol·L -1 It has a good linear relationship with the current of the screen electrode test paper within the range, and the detection limit is 4.6 mmol·L -1 .

Embodiment 3

[0020] Example 3 Response of different batches of screen-printed electrode test paper to β-hydroxybutyric acid

[0021] First, accurately weigh 0.198 g of β-hydroxybutyric acid and dissolve it in 10 mL of PBS solution (containing 0.1 mol L -1 KClO 4 ), the concentration is 0.27 mmol·L -1 , 10 different batches of electrodes were used to measure the current response of β-hydroxybutyric acid on the screen-printed electrode test paper at a potential of 0.4 V, such as image 3 shown.

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Abstract

The invention relates to the field of rapidly diagnosing diabetic ketoacidosis. Electrochemical test paper is prepared by using a silk-screen printing process; and the content of beta-hydroxybutyrate in human blood is detected by current which is generated when beta-hydroxybutyrate is oxidized by an enzyme layer on the test paper, so as to judge whether the diabetic ketoacidosis exists or not. The enzyme layer is prepared from nitrocellulose, hydroxybutyrate dehydrogenase, glutaraldehyde, potassium ferricyanide, reduced coenzyme and chitosan. The preparation method of the enzyme layer comprises the following steps of: dissolving nitrocellulose, hydroxybutyrate dehydrogenase, potassium ferricyanide, reduced coenzyme and chitosan with a phosphoric buffered solution; then reacting with glutaraldehyde; dropwise adding a mixed solution on the test paper; and drying to finally obtain the enzyme layer of the electrochemical test paper for detecting the beta-hydroxybutyrate. The silk-screen printing electrode provided by the invention can be widely applied to rapid diagnosis of diabetic ketoacidosis.

Description

technical field [0001] The invention relates to the preparation of a screen-printed electrode test paper for rapidly diagnosing diabetic ketoacidosis, in particular to an electrochemical test paper capable of detecting beta-hydroxybutyric acid. Background technique [0002] Diabetic ketoacidosis (DKA): An acute complication of diabetes. Acidosis caused by a severe shortage of insulin caused by a sharp rise in blood sugar. Ketone bodies are due to the aggravation of metabolic disorders in diabetic patients, accelerated fat movement and decomposition, and a large amount of fatty acids are produced by β-oxidation in the liver. When the excessive production of ketone bodies exceeds the extrahepatic self-oxidative capacity, the increase of blood ketone bodies and the increase of urinary ketone body elimination are called ketosis. The blood ketone body is further increased, and the metabolic disorder is intensified, so that the excessive generation of organic acids consumes the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 陈芳军
Owner 湖南尔文水电建材有限公司
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