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Method for producing 1,3-propylene glycol by using microbial fermentation

A microbial fermentation, propylene glycol technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of good control and wide application

Inactive Publication Date: 2012-08-15
杭玉玲
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of sodium salts of organic acids is seldom. As a by-product of 1,3-propanediol fermentation production, the whereabouts of these mixed organic salts is a problem that must be solved in industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The method for microbial fermentation to produce 1,3-propanediol is to use glycerol as a raw material, use Klebsiella pneumoniae to ferment 1,3-propanediol, and use liquid ammonia to adjust the pH value of the fermentation liquid during the fermentation process. Specifically include the following steps:

[0025] (1) After the fermentation medium is sterilized, the bacteria are inserted into the fermentation tank for fermentation, the pH value of the medium is 6.5, and the fermentation temperature is 32°C;

[0026] (2) During the fermentation process, 0.1VVM compressed air was introduced, the stirring speed of the fermenter was 80rpm, and the pressure of the fermenter was kept at 0.01MPa. Glycerin was added after 3 hours of fermentation, and the rate of glycerin was 5g / L*h;

[0027] (3) During the fermentation process, when the pH value of the fermentation broth drops to 6.5, the pH value of the fermentation broth is adjusted by adding liquid ammonia to maintain the pH v...

Embodiment 2

[0031] The method for microbial fermentation to produce 1,3-propanediol is to use glycerol as a raw material, use Klebsiella acidogens to ferment 1,3-propanediol, and use liquid ammonia to adjust the pH value of the fermentation liquid during the fermentation process. Specifically include the following steps:

[0032] (1) After the fermentation medium is sterilized, the bacteria are inserted into the fermentation tank for fermentation, the pH value of the medium is 8.5, and the fermentation temperature is 38°C;

[0033] (2) During the fermentation process, 0.8VVM compressed air was introduced, the stirring speed of the fermenter was 250rpm, and the pressure of the fermenter was maintained at 0.07MPa. Glycerin was added after 5 hours of fermentation, and the rate of glycerol was 50g / L*h;

[0034] (3) During the fermentation process, when the pH value of the fermentation broth drops to 6.5, the pH value of the fermentation broth is adjusted by adding liquid ammonia to maintain t...

Embodiment 3

[0038] The method for microbial fermentation to produce 1,3-propanediol is to use glycerol as a raw material, use Klebsiella pneumoniae to ferment 1,3-propanediol, and use liquid ammonia to adjust the pH value of the fermentation liquid during the fermentation process. Specifically include the following steps:

[0039] (1) After the fermentation medium is sterilized, the bacteria are inserted into the fermentation tank for fermentation, the pH value of the medium is 7.0, and the fermentation temperature is 35°C;

[0040] (2) During the fermentation process, 0.5VVM of compressed air was introduced, the stirring speed of the fermenter was 160rpm, and the pressure of the fermenter was maintained at 0.04MPa. Glycerin was added after 4 hours of fermentation, and the rate of glycerin was 30g / L*h;

[0041] (3) During the fermentation process, when the pH value of the fermentation broth drops to 6.5, the pH value of the fermentation broth is adjusted by adding liquid ammonia to mainta...

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PUM

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Abstract

The invention provides a method for producing 1,3-propylene glycol by using microbial fermentation. The method takes glycerol as a raw material and uses microbial fermentation to produce 1,3-propylene glycol, wherein the used microbe strain is one of Klebsiella pneumoniae, Klebsiella acid-producing bacillus or clostridia butyricum, and liquid ammonia is used for adjusting the pH value of a fermentation liquid in the fermentation process. By using the method, the pH value in the fermentation process can be well controlled, and the by-product obtained by the reaction has wide application.

Description

technical field [0001] The invention relates to a method for producing 1,3-propanediol by microbial fermentation, belonging to the field of biochemical synthesis. Background technique [0002] The existing method for producing 1,3-propanediol by microbial fermentation is to use glycerol as a raw material to produce 1,3-propanediol by microbial fermentation, and the strains used include Klebsiella pneumoniae and Klebsiella acidogens Or any one of Clostridium butyricum, etc., these bacteria have the ability to convert glycerol into 1,3-propanediol under anaerobic or microaerobic conditions. Regardless of the bacterial species, the general fermentation medium is mainly glycerin and water, and is equipped with inorganic salts such as dipotassium hydrogen phosphate, potassium dihydrogen phosphate, and ammonium sulfate. [0003] 2,3-butanediol and some organic acids, such as succinic acid, lactic acid, acetic acid, etc., can also be obtained when 1,3-propanediol is fermented by e...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/22C12R1/145
Inventor 杭玉玲
Owner 杭玉玲
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