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Method for pretreating enramycin fermentation liquor and method for preparing enramycin premix by using enramycin fermentation liquor

A technology of enramycin and fermentation liquid, which is applied in the field of preparation of enramycin premix, can solve the problems of poor stability and reduced enramycin degradation degree, and achieve convenient application process, low cost and low degradation The effect of reducing

Active Publication Date: 2012-08-15
山东胜利生物工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the problem that the finished product of enramycin premix is ​​not stable, the present invention provides a method for pretreatment of fermented liquid of enramycin, which is simple and easy to implement and low in cost. The degradation degree of enramycin was significantly reduced

Method used

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  • Method for pretreating enramycin fermentation liquor and method for preparing enramycin premix by using enramycin fermentation liquor

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Experimental program
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Effect test

Embodiment 1

[0015] Get 2 batches of fermented broths that have been fermented, and process them as follows: 1. Control group: no treatment; 2. Experimental group 1: adjust the pH value of the fermented broth to 5.0 with phosphoric acid, and then add 0.9% aluminum sulfate of the fermented broth quality; 3. Experimental group 2: Use hydrochloric acid to adjust the pH value of the fermentation broth to 5.0, and then add 0.9% aluminum sulfate in the mass of the fermentation broth; 4. Experimental group 3: Use citric acid to adjust the pH value of the fermentation broth to 5.0, and then add 0.9% of the mass of the fermentation broth of aluminum sulfate. Then, under the same conditions, the fermentation broth was subjected to plate-and-frame filtration (pressure 0.6MPa, time 2h), and the filter cake was flash-dried (inlet air temperature 100°C, outlet air temperature 80°C), and the obtained enramycin mycelium was dried The body is dried and pulverized into enramycin fine powder (80-100 mesh), a...

Embodiment 2

[0019] 1. Fermentation broth pretreatment:

[0020] Adjust the pH value of the enramycin fermentation broth with phosphoric acid so that the pH value of the fermentation broth is in the range of 4-5, and then add aluminum sulfate with a quality of 0.9% of the fermentation broth to prepare for extraction;

[0021] 2. Extraction of enramycin:

[0022] The fermentation broth is filtered with a plate and frame filter, the pressure of the plate and frame is 0.6MPa, press filter for 2 hours, the temperature of the inlet air is controlled at 90-95°C, the temperature of the material is 70-80°C, and the filter cake is flash-dried , to obtain the dried product of enramycin mycelium, the dried product is pulverized into 80-100 mesh enramycin fine powder, finally, adding auxiliary materials such as rice husk powder and zeolite powder to the fine powder to adjust its concentration, and mixing uniformly to obtain Enramycin premix finished product, the storage stability of the resulting fin...

Embodiment 3

[0024] 1. Fermentation broth pretreatment:

[0025] Phosphoric acid is used to adjust the pH value of the enramycin fermentation broth, so that the pH value of the fermentation broth is in the range of 5.0-6.0, and then adding aluminum sulfate with a mass of 0.9% of the fermentation broth to prepare for extraction;

[0026] 2. Extraction of enramycin:

[0027] The fermented liquid is filtered with a plate and frame filter. The pressure of the plate and frame is 0.5MPa, press filter for 2 hours, control the inlet air temperature at 105-110°C, and the material temperature at 85-90°C, and flash dry the filter cake. Obtain the dried product of enramycin mycelium, crush the dried product into 80-100 mesh enramycin fine powder, and finally add auxiliary materials such as rice husk powder and zeolite powder to the fine powder to adjust its concentration, mix evenly, and obtain Lamycin premix finished product, the storage stability of gained finished product has improved by 12.6%.

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Abstract

The method discloses a method for pretreating an enramycin fermentation liquor. The method comprises the following steps of: before extracting enramycin from the enramycin fermentation liquor, firstly adjusting the pH value of the fermentation liquor to a range of 4.0 to 7.0 by using an acid, and then adding aluminum sulfate accounting for 0.8-1.0% the weight of the fermentation liquor. The invention also discloses a method for preparing an enramycin premix. The method, compared with the existing enramycin production technique, is mainly characterized by pretreating the fermentation liquor of the enramycin so as to improve the quality of the finished product of the enramycin premix. The method for preparing the enramycin premix disclosed by the invention is simple and easy to implement and low in cost; and compared with the finished product without being subjected to pretreatment, the degradation degree of the enramycin obtained through the pretreated fermentation liquor is obviously reduced and the stability of the obtained enramycin premix finished product in the storage process is obviously improved. Furthermore, by adopting the method for preparing the enramycin premix disclosed by the invention, the enramycin finished product is more convenient and stable for storing, transporting and applying.

Description

technical field [0001] The invention relates to a pretreatment method of enramycin fermentation liquid and a method for preparing enramycin premix. Background technique [0002] Enramycin (enramycin), also known as enramycin, enramycin, enramycin and duramycin, is a polypeptide antibiotic. Initially in 1966, researchers from Japan's Takeda Pharmaceutical Co., Ltd. isolated a fermentation metabolite produced by a strain of fungicidal Streptomyces fungicidious NO.B-5477 from the soil of Nishinomiya City, Hyogo Prefecture, Japan. In 1974, the drug was officially registered in Japan, and has since been registered and widely used in many countries. In 1993, Japan's Takeda Pharmaceutical Co., Ltd. applied to the Ministry of Agriculture of China to register the drug, and the registered name was Enramycin Premix (Enlading), which was used as a drug feed additive. In 2000, Schering-Plough acquired Japan's Takeda Animal Health Company. This product is exclusively produced and operat...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K1/36C07K1/34C07K1/30C12P21/02A23K1/17C12R1/465A23K20/195
Inventor 张翠芬王建丽王向前贾同宽张子臣
Owner 山东胜利生物工程有限公司
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