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BmCP274 promoter of bombyx mori cuticular protein, recombinant expression vector and application of the promoter

A technology of epidermal protein and expression vector, applied in the biological field, can solve problems such as incomplete elucidation and incomparable mechanical properties

Active Publication Date: 2012-07-25
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The formation of silk fibers in living organisms is an extremely complex process that has not been fully elucidated so far
Existing synthetic fibers are artificially simulated in the natural silk production process of insects and synthesized in vitro, but their mechanical properties are far from being compared with spider silk fibers

Method used

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  • BmCP274 promoter of bombyx mori cuticular protein, recombinant expression vector and application of the promoter
  • BmCP274 promoter of bombyx mori cuticular protein, recombinant expression vector and application of the promoter
  • BmCP274 promoter of bombyx mori cuticular protein, recombinant expression vector and application of the promoter

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Embodiment Construction

[0015] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0016] The silkworm species used in the examples is Dazao, provided by the Silkworm Gene Resource Bank of Southwest University of China.

[0017] 1. Cloning of Bombyx mori epidermal protein BmCP274 promoter

[0018] According to the sequence nscaf1681 in the silkworm genome database, a pair of upstream and downstream primers were designed. The designed primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. The primer sequences are as follows:

[0019] 274-F: 5'-ttgtcgacgtagcct...

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Abstract

The invention discloses a BmCP274 promoter of bombyx mori cuticular protein. The promoter consists of nucleotide sequences shown in SEQ ID No.1, can drive foreign proteins to specifically express at a front silk gland of the bombyx mori and can be used as a specific promoter at the front silk gland of the silkworm. The invention further discloses a recombinant expression vector containing the promoter, which provides a powerful tool for a next-step study on regulating ion channel protein expression at the front silk gland of the silkworm to affect the behavior of the bombyx mori and improve the performance of the silk fiber.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a tissue-specific promoter, its expression vector and its application. Background technique [0002] The silk fiber produced by the silkworm under natural conditions is a fiber material widely used in textile industry, biomedicine and other fields. Spiders and silkworms have similar spinning bodies, but the silk fibers produced by them have stronger hardness and better toughness than silkworm silk. The formation of silk fibers in living organisms is an extremely complicated process, which has not been fully elucidated so far. Existing synthetic fibers are artificially simulated in the natural silk production process of insects and synthesized in vitro, but their mechanical properties are far from being comparable to spider silk fibers. If the silkworm can be efficiently and specifically produced a large amount of high-quality spider silk-like fibers, it will be of great significance ...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/866
Inventor 夏庆友衣启营赵萍王鑫马三垣徐汉福
Owner SOUTHWEST UNIVERSITY
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