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Microalgae cell flocculating and harvesting technology

A microalgae cell and flocculation technology, applied in the direction of single-cell algae, microorganism-based methods, microorganisms, etc., can solve the problems of low flocculation efficiency and high cost of flocculants, and achieve the advantages of low price, convenient flocculation process operation, and improved flocculation efficiency. Effect

Inactive Publication Date: 2012-07-25
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the above invention patents and documents, the cost of flocculants is high and the flocculation efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] According to mass ratio, chitosan: the ratio of attapulgite=1:10, take 0.3g chitosan and add in 10mL, 5mol / L hydrochloric acid, after mixing and stirring for a period of time, add 3g attapulgite wherein, normal temperature After stirring for 24 hours, dry at 75°C, grind, and pass through a 200-mesh sieve to obtain a flocculant.

[0019] First, the flocculant and coagulant CaCl 2 The solutions are mixed, and then added to the suspension of Chlorella vulgaris, so that the concentration of the flocculant in the Chlorella culture solution is 0.4g / L, and the coagulation aid CaCl 2 The concentration is 0.02g / L. Adjust the pH of the microalgae culture solution to 7-9, stir the chlorella suspension at a speed of 300rpm for 5 minutes, let the chlorella culture solution stand for 30 minutes, and the degree of flocculation is above 99%. Clear liquid, collect flocs.

Embodiment 2

[0021] According to the mass ratio, melamine: attapulgite=1:10 ratio, weigh 0.3g melamine and add it to 10mL, 5mol / L hydrochloric acid, after mixing and stirring for a period of time, add 3g attapulgite to it, and stir at room temperature for 24h, Dry at 75°C, grind, and pass through a 200-mesh sieve to obtain a flocculant.

[0022] First, the flocculant and coagulant CaCl 2 The solution is mixed, and then added to the suspension of Chlorella vulgaris, so that the concentration of the flocculant in the Chlorella culture solution is 0.37g / L, and the coagulation aid CaCl 2 The concentration is 0.025g / L. Adjust the pH of the microalgae culture solution to 7-9, stir the chlorella suspension at a speed of 400rpm for 5 minutes, let the chlorella culture solution stand for 30 minutes, and the degree of flocculation is above 95%. Clear liquid, collect flocs.

Embodiment 3

[0024] According to the mass ratio, EDTA: attapulgite = 1:10 ratio, weigh 0.3g EDTA and add it to 10mL, 5mol / L hydrochloric acid, mix and stir for a period of time, then add 3g attapulgite to it, and stir at room temperature for 24h, Dry at 75°C, grind, and pass through a 200-mesh sieve to obtain a flocculant.

[0025] First, the flocculant and coagulant CaCl 2 The solution is mixed, and then added to the suspension of Chlorella vulgaris, so that the concentration of the flocculant in the Chlorella culture solution is 0.5g / L, and the coagulation aid CaCl 2 The concentration is 0.02g / L. Adjust the pH of the microalgae culture solution to 7-9, stir the chlorella suspension at a speed of 300rpm for 8 minutes, let the chlorella culture solution stand for 30 minutes, and the degree of flocculation is above 99%. Clear liquid, collect flocs.

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Abstract

The invention relates to a microalgae cell flocculating and harvesting technology, belonging to the technical field of energy microalgae. The technology is characterized in that chitosan, melamine or EDTA (ethylene diamine tetraacetic acid) and attapulgite are prepared into a flocculating agent; and CaCl2 is added as a flocculation aid to perform flocculating and harvesting of microalgae. The invention provides preparation of a flocculating agent and a flocculating and harvesting technology. The technology comprises the following steps of: drying the flocculating agent, grinding and sieving; adding the flocculating agent into the microalgae culture solution so that the concentration of the flocculating agent is 0.3-0.6g / L, and the concentration of the flocculation aid CaCl2 is 0.01-0.03g / L; adjusting the microalgae culture solution until the pH is 7-9; and stirring uniformly and standing for precipitation, wherein the flocculation degree can be more than 99%. For the flocculating technology provided by the invention, the raw materials are cheap; and the technology is safe and environment-friendly and convenient to operate, has high flocculation efficiency, and can be widely used for harvesting microalgae in the industrial production.

Description

technical field [0001] The invention belongs to the technical field of energy microalgae, and in particular relates to a technique for flocculating and collecting microalgae by using chemical substances to modify natural substances. Background technique [0002] Microalgae are widely distributed, with strong environmental adaptability and short growth cycle. They are a new type of low-carbon environmental protection resources and play a very important role in the production and life of human society. The oil content in microalgae is high, and some polyunsaturated fatty acid components are necessary for life. The traditional way of obtaining unsaturated fatty acids, such as extracting from plant seed oils such as evening primrose and blackcurrant, or extracting from marine animals such as crustaceans and fish, has been unable to meet market demand. Microalgae has the unique advantages of fast growth, no restrictions on raw materials, production areas and seasons, and the com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 许伟邵荣吴乔林余晓红
Owner YANCHENG INST OF TECH
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