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Preparation method of mulberry silk tissue engineering scaffold

A technology of tissue engineering scaffold and mulberry silk, which is applied in medical science, prosthesis, etc., can solve the problems of low porosity of scaffold model and inability to make models

Inactive Publication Date: 2014-07-09
XUZHOU NAILI MACROMOLECULE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional method of using models to make brackets has problems such as inability to make arbitrary complex models, often unable to meet the needs of various bracket models, and low porosity. Low temperature forming technology can solve these problems well

Method used

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  • Preparation method of mulberry silk tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] 1) Directly design with the help of 3D CAD software or use solid reverse engineering to collect the geometric shape and structural information of the prototype to obtain a 3D model.

[0024] 2) According to the above-mentioned three-dimensional model and the specific needs of the bracket, perform layering to obtain the data file formed by the low-temperature forming machine. In the layering, set the layer thickness to 0.19mm, the distance between wires to 1.3mm, and the scanning angle to 0° / 90°.

[0025] 3) slurry preparation, specifically comprising the following steps:

[0026] 3-1) Silk degumming: use 50 times the volume mass fraction of 0.5% Na 2 CO 3 solution, refine the silk twice at a temperature of 95-100° C., each time for 30-40 minutes, and rinse with deionized water for 2-3 times.

[0027] 3-2) Silk dissolution: Dissolve 10g of degummed silk in boiling 100mL CaCl with a mass fraction of 40% 2 solution, the dissolving time is 60-80 minutes, and the silk fib...

example 2

[0034] 1) Directly design with the help of 3D CAD software or use solid reverse engineering to collect the geometric shape and structural information of the prototype to obtain a 3D model.

[0035] 2) According to the above-mentioned three-dimensional model and the specific needs of the bracket, layering is carried out to obtain the data file formed by the low-temperature forming machine. In the layering, the layering thickness is set to 0.17mm, the distance between wires is 1.2mm, and the scanning angle is 0° / 90°.

[0036] 3) slurry preparation, specifically comprising the following steps:

[0037] 3-1) Silk degumming: use 50 times the volume mass fraction of 0.5% Na 2 CO 3 solution, refine the silk twice at a temperature of 95-100° C., each time for 30-40 minutes, and rinse with deionized water for 2-3 times.

[0038] 3-2) Silk dissolution: Dissolve 10g of degummed silk in boiling 100mL CaCl with a mass fraction of 40% 2 solution, the dissolving time is 60-80 minutes, and...

example 3

[0045] 1) Directly design with the help of 3D CAD software or use solid reverse engineering to collect the geometric shape and structural information of the prototype to obtain a 3D model.

[0046] 2) According to the above-mentioned three-dimensional model and the specific needs of the bracket, layering is carried out to obtain the data file formed by the low-temperature forming machine. In the layering, the layering thickness is set to 0.15mm, the distance between wires is 1.2mm, and the scanning angle is 0° / 90°.

[0047] 3) slurry preparation, specifically comprising the following steps:

[0048] 3-1) Silk degumming: use 50 times the volume mass fraction of 0.5% Na 2 CO 3 solution, refine the silk twice at a temperature of 95-100° C., each time for 30-40 minutes, and rinse with deionized water for 2-3 times.

[0049] 3-2) Silk dissolution: Dissolve 10g of degummed silk in boiling 100mL CaCl with a mass fraction of 40% 2 solution, the dissolving time is 60-80 minutes, and...

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Abstract

The invention relates to a preparation method of a mulberry silk tissue engineering scaffold. Artificially synthesized macromolecular material PLGA (polylactic-co-glycolic acid) is adopted in the traditional method, degradation products of the PLGA are acid, local pH value is reduced and inflammation can be easily caused. The preparation method provided by the invention comprises that mulberry silk is taken as a raw material and is dissolved with CaCl2 solution with the mass fraction of 40% after being refined by Na2CO3 solution with the concentration of 0.5g / dL, then filtering is carried out by adopting a dialysis bag, the obtained fibroin protein solution is added into mixed solution of chitosan / acetic acid, stirring to be uniform is carried out, then mixture is added into a charging barrel of a sprayer of forming equipment, low-temperature forming is carried out, a freezing scaffold is placed into a freeze drying machine, freeze drying is carried out, and a scaffold which is in solid state at normal temperature is obtained. The degradation products of the mulberry silk tissue engineering scaffold prepared by the preparation method provided by the invention are polypeptides, local pH value can not be reduced, and inflammation can not be caused, thus the mulberry silk tissue engineering scaffold is harmless to human body.

Description

technical field [0001] The invention belongs to the technical field of medical tissue engineering and low-temperature forming, and in particular relates to a preparation method of a mulberry silk tissue engineering scaffold. Background technique [0002] The term tissue engineering was first proposed by the National Science Foundation (NASF) at the Bioengineering Conference in 1987. Specifically, it includes three important factors: scaffold materials, seed cells and culture methods. The main step is to cultivate and proliferate target tissue cells , plant high-concentration cells on the scaffold material, cultivate and expand the seed cells on the scaffold material, and implant the tissue constructed in vitro into the patient's body. With the gradual degradation and absorption of the biological scaffold material, the cells continue to proliferate and secrete cells The extracellular matrix eventually forms living tissue with normal structure and function. As an artificial e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22
Inventor 史廷春安晓郑文祥王瑞艳张毅
Owner XUZHOU NAILI MACROMOLECULE TECH CO LTD
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