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Establishment method and application of zebrafish multiple sclerosis model

A technique for multiple sclerosis and establishment of methods, applied in the field of nerve myelin damage and establishment of zebrafish multiple sclerosis models, can solve the problem that treatment is not necessarily effective, and achieves strong intuition, strong comparability, and experimental analysis. simple method effect

Active Publication Date: 2012-07-25
HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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Problems solved by technology

However, the transgenic fluorescent zebrafish is a normal myelin zebrafish, which is completely different from the pathological changes of demyelination in MS. Therefore, the myelin formation promoter screened by the normal transgenic fluorescent zebrafish with myelin is not necessarily effective for the treatment of MS. efficient

Method used

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  • Establishment method and application of zebrafish multiple sclerosis model
  • Establishment method and application of zebrafish multiple sclerosis model
  • Establishment method and application of zebrafish multiple sclerosis model

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Embodiment Construction

[0068] The purpose of the present invention is to provide a method for establishing a living zebrafish multiple sclerosis model, and at the same time provide a method for using the model to screen multiple sclerosis therapeutic drugs and evaluate compound-induced myelin damage. The method provided by the invention has the advantages of simplicity, rapidity, economy, high efficiency, high throughput and the like.

[0069] One, the present invention provides a kind of establishment method of live zebrafish multiple sclerosis model, design scheme is:

[0070] 1 zebrafish selection

[0071] Take 4 to 5 pairs of zebrafish parents to mate, according to Westerfield [33] method for hatching embryos. Put the zebrafish at the optimal stage of treatment under a dissecting microscope, pick the zebrafish with normal development and move them into 6, 12, 24, 48, 96 or 384-well microplates, and put different numbers according to the microplate specifications zebrafish.

[0072] 2 compoun...

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Abstract

The invention relates to an establishment method for a zebrafish multiple sclerosis model, and a method for screening therapeutic medicines for multiple sclerosis or evaluating compound-induced nerve myelin injuries by using the animal model. The establishment method of the zebrafish multiple sclerosis model mainly comprises the following steps of: zebrafish selection, compound treatment, whole zebrafish immunofluorescent staining, image analysis and / or microplate selection, and statistical analysis. The method disclosed by the invention has the advantages of being economic, simple and convenient, rapid, efficient, less in the dosage of compound and the like, can realize a purpose of screening the therapeutic medicines for multiple sclerosis with a high flux in vivo, and is significant for acceleration for the research and development process of the therapeutic medicines for multiple sclerosis, and therapy for patients with multiple sclerosis.

Description

technical field [0001] The invention belongs to the field of drug screening (evaluation), in particular to a method for establishing a simple, economical and high-throughput zebrafish multiple sclerosis model, and using the animal model to screen multiple sclerosis therapeutic drugs or evaluate compound-induced Methods of nerve myelin damage. Background technique [0002] Multiple sclerosis (MS) is an autoimmune disease characterized by white matter demyelination of the central nervous system. It may be a neuroimmune process caused by genetically susceptible individuals and environmental factors. Due to its high incidence , becoming one of the most important neurological diseases with a chronic course and a predisposition to young adults [1] . Epidemiology shows that the disease has about 400,000 patients in North America, about 65,000 patients in my country, and more than 1 million patients worldwide. There are more women than men, and most of them are in the 20-40 age gr...

Claims

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Application Information

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IPC IPC(8): A61K31/473A61P37/02G01N21/64
Inventor 李春启朱晓宇朱凤郭胜亚
Owner HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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