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Ionizing radiation degradation method for sea cucumber polysaccharide

The technology of sea cucumber polysaccharide and ionizing radiation is applied in the field of degradation method of polysaccharide compounds, which can solve the problems of detachment of sulfate group and branched-chain sulfated fucose, complicated operation of oxidative decomposition method, difficult process control, etc. Loss, product molecular weight uniformity, no environmental pollution effect

Active Publication Date: 2015-04-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previously, Yutaka Kariya (JBiochem 2002, 132, (2), 335-43) and Paulo Mourao (J Biol Chem 1996, 271, (39), 23973-84) and other research groups used dilute acid method to prepare oligosaccharides. , easily lead to the shedding of sulfate groups and branched fucose sulfates, affecting related activities
However, the oxidative decomposition method is cumbersome to operate, the process is difficult to control, and the reproducibility is poor.

Method used

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  • Ionizing radiation degradation method for sea cucumber polysaccharide
  • Ionizing radiation degradation method for sea cucumber polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: The ionizing radiation degradation method of sea cucumber polysaccharide:

[0023] Weigh four parts of Philippine sea cucumber chondroitin sulfate (50 mg each) and prepare them into aqueous solutions with mass percentage concentrations of 1%, 2%, 5%, and 10%. All were treated with ionizing radiation, the absorbed dose was 50kGy, and the radiation dose rate was 45kGy / min; after irradiation, it was allowed to stand for 1.5 hours, the non-degradable matter settled to the bottom of the container, and the supernatant was taken.

[0024] The four supernatants were vacuum freeze-dried (-40° C.) to obtain 42 mg, 39 mg, 40 mg, and 35 mg of low molecular weight chondroitin sulfate (ie, low molecular weight oligosaccharides).

[0025] HPLC high performance gel permeation chromatography (TSK3000, 4000) was used to determine the relative molecular weight, number average molecular weight, weight average molecular weight, and molecular weight distribution index of different concen...

Embodiment 2

[0029] Example 2: The ionizing radiation degradation method of sea cucumber polysaccharide:

[0030] Weigh 5 parts of Philippine sea cucumber chondroitin sulfate (50 mg each), and prepare them into an aqueous solution with a mass percentage concentration of 1%. They were treated with 5 different doses of ionizing radiation, the absorbed doses were 10KGy, 20KGy, 50KGy, 100KGy, 150KGy, and the radiation dose rate was 45kGy / min; 1.5 hours after the irradiation, the non-degradable matter settled to the bottom of the container, and take it Clear liquid.

[0031] The five supernatants were vacuum freeze-dried (-40°C) to obtain 39 mg, 38 mg, 42 mg, 41 mg, and 37 mg of low-molecular-weight chondroitin sulfate (ie, low-molecular-weight oligosaccharides).

[0032] HPLC high performance gel permeation chromatography (TSK3000) was used to determine the relative molecular weight of the same concentration of chondroitin sulfate under different irradiation doses. Details are shown in Table 2.

[0...

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Abstract

The invention discloses an ionizing radiation degradation method for sea cucumber polysaccharide. The method sequentially comprises the following steps of: 1) completely dissolving the sea cucumber polysaccharide into distilled water to obtain an aqueous solution of the sea cucumber polysaccharide; 2) performing ionizing radiation degradation on the aqueous solution of the sea cucumber polysaccharide at normal temperature by adopting 60 Co, wherein the absorbed dose is 2 to 200kGy; and 3) standing and settling or centrifuging the obtained product in the 2) to remove non-degradable settlement and obtain a clear solution, drying the clear solution, and thus obtaining an oligosaccharide with low molecular weight. The product obtained by adopting the method has uniform and centralized molecular weight, the molecular weight distribution index is close to 1, and the product has good uniformity.

Description

Technical field [0001] The invention relates to a degradation method of polysaccharide compounds, in particular to a method for ionizing radiation to degrade sea cucumber polysaccharides. Background technique [0002] Sea cucumber polysaccharides mainly include sea cucumber chondroitin sulfate (SC-CHS) (J Biol Chem 1996, 271, (39), 23973-84; J Biol Chem 1988, 263, (34), 18176-83; Journal of Biological Chemistry 1991, 266, (21), 13530-13536) and sea cucumber fucoidan sulfate (SC-FUC) (Carbohydr Res 1994, 255, 225-40) two polysaccharides, of which SC-CHS is derived from sea cucumber A polysaccharide extracted from the body wall can be derived from a variety of sea cucumbers. Although its chemical structure is different, its biological activity is similar. Yutaka Kariya (J Biochem 2002, 132, (2), 335-43) extracts SC-CHS from Japanese sea cucumber, and the analysis of sugar composition shows that fucose (Fuc): acetylgalactosamine (GalNAc): glucose Aldehydic acid (GlcA): sulfate (SO...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 陈士国吴念刘东红胡亚芹陈建初孙玉敬吴丹叶兴乾
Owner ZHEJIANG UNIV
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