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Extraction method for high-purity tetrodotoxin

A technology of tetrodotoxin and extraction method, which is applied in nervous system diseases, drug combination, organic chemistry, etc. It can solve the problems of cumbersome pre-treatment, difficulty in avoiding corruption of liquid medicine and medicine dregs, and difficulty in realizing industrialization, etc.

Inactive Publication Date: 2012-07-18
王秀菊 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although measures such as low-temperature soaking can be adopted, it is difficult to avoid the phenomenon of corruption of the medicinal liquid and medicinal residue
In the carbon column adsorption method, the pretreatment of carbon is relatively cumbersome, and there is a defect that carbon can irreversibly adsorb tetrodotoxin
In addition, the large-scale membrane filtration method has a large demand for funds, and it is not easy to realize industrialization

Method used

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  • Extraction method for high-purity tetrodotoxin

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Embodiment Construction

[0014] A kind of extraction method of high-purity tetrodotoxin of the present invention comprises the following processing steps:

[0015] a. Stock solution preparation:

[0016] Cut puffer fish roe or liver into pieces or strips, add 1-2 times of deionized water, place in ultrasonic for 1-2 hours, 3-5 times in a row, until no tetrodotoxin is detected. The material liquid after ultrasonic is placed in the water shaker, and the material liquid is thrown out. Combine and shake off the feed liquid, quickly boil for 5-15 minutes, and quickly cool down, filter out solids such as protein with a filter cloth of 300-400 mesh, degrease the feed liquid with methylene chloride, and then use filter paper and micro Pore ​​filter membrane for two-stage filtration, plate and frame through the liquid transfer ion exchange system for standby.

[0017] b. Ion exchange:

[0018] Put the stock solution filtered by the plate and frame in step a on the ion exchange column for ion exchange, start...

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Abstract

The invention discloses an extraction method for high-purity tetrodotoxin, which is mainly used for extracting tetrodotoxin from animal feed puffer fish seeds and livers by jointly utilizing the methods of ultrasonic fast dissolving out, organic solvent derosination, plate frame multi-level filtration, positive ion exchange resin, small film filtration, high-performance liquid preparation and the like and obtaining high-purity high-stability tetrodotoxin crystals. Tetrodotoxin has the effect of blocking a sodium ion channel is obvious in analgesic effect and free of dependency and is widely used in advanced cancer, postoperative pain relieving, de-addiction curing of drug abuse and the like. Extraction and preparation of high-purity tetrodotoxin can provide the possibility of developing a novel high-efficiency pain killer free of dependency.

Description

technical field [0001] The invention relates to the technical field of marine organisms, and mainly relates to extracting high-purity monomer substances from animal raw materials, in particular to an extraction method for obtaining high-purity tetrodotoxin from puffer fish roe and liver. Background technique [0002] Tetrodotoxin is a unique component of puffer fish, a marine migratory fish, and mainly exists in the ovary, liver and blood. Studies have shown that tetrodotoxin is also found in other fish, amphibians, mollusks, and echinoderms, such as tiger shrimp, blue-ringed octopus, California python, and even crustacean crabs. Tetrodotoxin is also known as round toxin, python toxin, oriental toxin and so on. Our country has a long history of studying tetrodotoxin, which was first recorded in "Shan Hai Jing", "Shen Nong's Materia Medica", "Kaibao Materia Medica", "Compendium of Materia Medica", etc. all have records about tetrodotoxin. [0003] Since ancient times, tetro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/22A61P25/04A61P25/36
Inventor 王秀菊丁亚民尹志勇
Owner 王秀菊
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