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Preparation method of acellular biomembrane dressing

A biofilm and decellularization technology, used in medical science, bandages, absorbent pads, etc., can solve the problems of biofilm damage, complicated operation, low efficiency, etc., and achieve high efficiency, short degreasing time, and light damage.

Active Publication Date: 2012-07-18
上海优越生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing decellularized biofilm materials have the following disadvantages: the operation is more complicated, the cycle is longer, the efficiency is low, and it is easy to cause damage to the biofilm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A kind of method for preparing decellularized biofilm dressing of the present invention, comprises the following steps:

[0016] a. Preparation process of membrane sheet: after the surface fat is removed from the biofilm, the biofilm is prepared into a mesh-like biofilm with a puncher, and the biofilm is the pleural peritoneum and small intestinal mucosa of the animal;

[0017] b. Virus inactivation process: The virus inactivation solution composed of peracetic acid with a mass concentration of 0.1% and sodium chloride with a mass concentration of 1.0% was used to inactivate the virus for 30 minutes, and the biofilm and the virus inactivation solution were separated. The mass ratio is 1: 3;

[0018] c. Degreasing process: use a mixed degreasing agent with a mass concentration of 1.0%% soda ash, a mass concentration of 1.0% caustic soda and a mass concentration of 0.1% Pingpingjia for degreasing for 30 minutes, and the mass ratio of the biofilm to the mixed degreasing ag...

Embodiment 2

[0023] A kind of method for preparing decellularized biofilm dressing of the present invention, comprises the following steps:

[0024] a. Preparation process of membrane sheet: after the surface fat is removed from the biofilm, the biofilm is prepared into a mesh-like biofilm with a puncher, and the biofilm is the pleural peritoneum and small intestinal mucosa of the animal;

[0025] b. Virus inactivation process: The virus inactivation solution composed of peracetic acid with a mass concentration of 0.1% and sodium chloride with a mass concentration of 1.0% was used to inactivate the virus for 30 minutes, and the biofilm and the virus inactivation solution were separated. The mass ratio is 1: 3;

[0026] c. Degreasing process: use a mixed degreasing agent with a mass concentration of 1.0%% soda ash, a mass concentration of 1.0% caustic soda and a mass concentration of 0.1% Pingpingjia for degreasing for 30 minutes, and the mass ratio of the biofilm to the mixed degreasing ag...

Embodiment 3

[0031] A kind of method for preparing decellularized biofilm dressing of the present invention, comprises the following steps:

[0032] a. Preparation process of membrane sheet: after the surface fat is removed from the biofilm, the biofilm is prepared into a circular hole-shaped biofilm with a puncher, and the biofilm is the pleural peritoneum and small intestinal mucosa of the animal;

[0033] b. Virus inactivation process: The virus inactivation solution composed of peracetic acid with a mass concentration of 0.5% and sodium chloride with a mass concentration of 3.0% was used to inactivate the virus for 45 minutes, and the biofilm and the virus inactivation solution were separated. The mass ratio is 1:4;

[0034] C, degreasing process: adopt the mixed degreasing agent that mass concentration 2.0% soda ash, mass concentration 2.0% caustic soda and mass concentration 0.5% sodium dodecylalanine form degreasing 45 minutes, the mass ratio of biofilm and mixed degreasing agent is...

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Abstract

The invention discloses a preparation method of an acellular biomembrane dressing. The acellular biomembrane is prepared from an animal biomembrane by viruses inactivating, degreasing, decellularizing, silver adding, lyophilizing, packaging and radiation disinfecting. The method has the advantages of short degreasing time, high efficiency, strong protection effect of biomembrane tissue structure, complete decellurarization, light damage to natural structure of the biomembrane, and antibacterial function.

Description

technical field [0001] The invention relates to a method for preparing an acellular biofilm dressing. Background technique [0002] Modern burn treatment has saved the lives of most severe burn patients, and biological dressings prepared from animal biofilms have been widely used on burn wounds. The decellularized biofilm removes the antigenic components of the biofilm, and after covering the wound, it has good compatibility with human tissue, can promote the growth of the wound, and induce tissue regeneration. The existing decellularized biofilm materials have the following disadvantages: the operation is complicated, the cycle is long, the efficiency is low, and the biofilm is easily damaged. Therefore, it is necessary to provide a new technical solution to solve the above problems. Contents of the invention [0003] The purpose of the present invention is to provide a method for preparing decellularized biofilm dressing with short period, high efficiency and light dam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40A61L15/44
Inventor 潘华倩潘银根
Owner 上海优越生物医学科技有限公司
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