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Application of Candida guilliermondii strain in elimination of patulin

A technology of patulin and candida, which can be used in applications, fungi, food preparation, etc., and can solve problems such as unsatisfactory effects.

Active Publication Date: 2013-09-25
FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mainly removed by physical methods such as adsorption and microwave treatment, but the effect is not very satisfactory, and it is urgent to develop new methods for the removal of patulin

Method used

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  • Application of Candida guilliermondii strain in elimination of patulin
  • Application of Candida guilliermondii strain in elimination of patulin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the influence of patulin on the growth of Candida guilliermondii (Candida guilliermondii) CGMCC No.2.63

[0026] 1. Activation of Candida guilliermondii CGMCC No.2.63 strain

[0027] Candida guilliermondii CGMCC No.2.63 stored at -20°C was streaked on NYDA plates. After culturing at 26°C for 72 hours, pick yeast cells from a well-growing single colony of Candida guilliermondii (Candida guilliermondii) and inoculate them into NYDB liquid medium for shaking and culturing for 24 hours at a culture temperature of 26°C and a shaker speed 200 rpm.

[0028] 2. Preparation of patulin standard stock solution

[0029] The patulin is a standard product of patulin, purchased from Qingdao Shenggong Company, and prepared into a mother solution with a concentration of 1 mg / ml when used. The preparation method is as follows: accurately weigh 10 mg of patulin standard substance, dissolve it in 10 ml of distilled water, fully stir to dissolve, and use a 0.22 μm microporo...

Embodiment 2

[0037] Embodiment 2, the detection of Candida guilliermondii CGMCC No.2.63 to the scavenging ability of patulin

[0038] 1. Activation of strains

[0039] With embodiment 1 step 1.

[0040] 2. Preparation of patulin standard stock solution

[0041] With embodiment 1 step 2.

[0042] 3. Preparation of culture medium containing different concentrations of patulin

[0043] With embodiment 1 step 3.

[0044] 4. Analysis of the scavenging ability of Candida guilliermondii CGMCC No.2.63 to patulin

[0045] Inoculate the activated yeast (Candida guilliermondii) CGMCC No.2.63 into the culture solution prepared in step 3 containing 50 μg / ml, 100 μg / ml and 200 μg / ml patulin respectively, so that the seasons after inoculation will also be covered with candida The cell concentration of yeast (Candida guilliermondii) was 2 × 10 5 cfu / ml, the culture temperature was 26°C, the shaker speed was 200 rpm, and each treatment was repeated 3 times. At the same time, a control group without ...

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Abstract

The invention discloses application of a yeast strain to elimination of patulin. The invention provides application of Candida guilliermondii to elimination of patulin. Experiments prove that Candida guilliermondii can quickly remove high concentration patulin in culture solution; patulin residues in three concentrations of culture solution can not be detected two days after the Candida guilliermondii cells with concentration being 2*105cfu / ml are inoculated into the culture solution containing 50mu g / ml of patulin, the culture solution containing 100mu g / ml of patulin and the culture solution containing 200mu g / ml of patulin respectively to be cultured. Application provided by the invention can provide a new solution to removal of patulin residues in such food as fruit juice.

Description

technical field [0001] The invention relates to the application of a yeast strain in eliminating patulin, in particular to the application of Candida guilliermondii in eliminating patulin. Background technique [0002] Patulin is a secondary metabolite of some Penicillium and Aspergillus fungi. It has extensive and strong toxic effects on humans and animals. Acute toxicity manifests as convulsions, convulsions, subcutaneous tissue edema, pulmonary and intestinal congestion, enteritis, anuria, and death; chronic toxicity can lead to gastric ulcers, immunosuppression, teratogenicity, and potential carcinogenicity, etc., and may also affect men of fertility. Patulin is soluble in water, ethanol, acetone, ethyl acetate and chloroform, slightly soluble in ether and benzene, insoluble in petroleum ether, and chemically stable under acidic conditions. [0003] Among the fungi capable of producing patulin, some fungi are plant pathogens, and the most important one is Penicillium ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23L2/84A23L2/02A23L1/015A23L5/20
Inventor 田世平李博强秦国政徐勇宗元元
Owner FUJIAN SANAN SINO SCI PHOTOBIOTECH CO LTD
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