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Rice cultivating method capable of improving resistant starch content

A technology of resistant starch and breeding methods, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems affecting the quality of life, the survival and development of human society, etc.

Inactive Publication Date: 2012-07-18
ZHEJIANG UNIV
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Problems solved by technology

[0003] Diabetes and other chronic diseases of the blood system are showing rapid growth, spreading to rural areas, and younger ages, which are increasingly affecting the survival and development of human society (the number one killer of life, seriously affecting the quality of life, and annual medical expenses. up to 100 billion yuan), and there is currently no cure

Method used

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  • Rice cultivating method capable of improving resistant starch content

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Mature embryos of rice variety R102 with an amylose content of 31.5% were used as explants, inoculated into the induction medium, and induced to form callus under the conditions of 25±1°C and light intensity of 3000 lux;

[0033] Take the callus induced to form for 2 weeks, transfer to the subculture medium, and subculture until the callus enters the doubling phase;

[0034] Take the doubly-proliferated callus, and pre-culture the recipient callus for 2 days at 25±1°C and under dark conditions protected from light;

[0035] Take the pre-cultured callus (about 300 petri dishes, containing more than 2000 pieces of callus), soak in the Agrobacterium suspension for 10 minutes, then discard the bacteria solution, dry it with sterile filter paper, and inoculate in On the co-culture medium, co-cultivate for 2 days at 25±1°C in dark conditions protected from light;

[0036] Take the above-mentioned co-cultured callus, wash and dry it, transfer it to the selection medium, and c...

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Abstract

The invention relates to a rice cultivating method capable of improving resistant starch content, which includes the steps: taking a mature embryo of a rice variety with amylase content larger than 25% as an explant, inducing callus, subculturing to a multiplication period, preculturing under the condition of dark, conducting agrobacterium co-culture transformation, transferring to a screening medium to conduct three turns of screening, taking resistant callus to be induced to differentiate seedlings, conducting histochemical staining on marked genes of a resistant plant, conducting molecular detecting on screened genes of the resistant plant, identifying differentiation little seedlings containing a target gene starch synzyme ssIII-2 insertion sequence, conducting detecting of the resistant starch content of hot rice on selected transgenosis plants, selecting transgenosis plants with the resistant starch content larger than 10%, continuously planting, evaluating expression stability of the resistant starch content, and propagating excellent strains with the resistant starch content larger than 10% stably. The rice with improved resistant starch content has significant meaning on conducting dietotherapy for diabetes patients.

Description

technical field [0001] The invention relates to a method for cultivating rice, in particular to a method for cultivating rice with improved resistant starch content. Background technique [0002] According to statistics from the Ministry of Health, there were about 92 million people with diabetes in my country in 2010, accounting for 9.3% of the total population in my country, and 142 million people with low glucose tolerance. [0003] Diabetes and other chronic diseases of the blood system are showing rapid growth, spreading to rural areas, and younger ages, which are increasingly affecting the survival and development of human society (the number one killer of life, seriously affecting the quality of life, and annual medical expenses. up to 100 billion yuan), and there is currently no cure. [0004] Medical research shows that: In addition to genetic factors, chronic diseases of the blood system such as diabetes are mainly caused by daily living habits such as daily diet,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张宁吴殿星舒小丽
Owner ZHEJIANG UNIV
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