Myoglobin assay kit
A technology for detecting kits and myoglobin, which is applied in the field of medical immunology, can solve the problems of increasing the amount of antibodies and production costs, false positive test results, and low accuracy, so as to improve accuracy and reliability and reduce antibody production. The effect of dosage and reduction of production cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] The present invention is further described in detail below through examples, but the present invention is not limited only to the following examples. The preparation of embodiment 1MB detection kit
[0029] The kit of the present invention relates to the main raw materials of reagents as follows:
[0030] 1. MB antibody: polyclonal antibody (commercially available).
[0031] 2. Latex: The present invention only exemplarily uses polystyrene latex particles (commercially available) with a diameter of 150-250 nm and carboxyl groups to carry out experiments.
[0032] The preparation of the main reagent of this embodiment is as follows:
[0033] Reagent R1: Glycine buffer solution containing 1.5% PEG6000 (polyethylene glycol 6000), 95 mmol / L NaCl, this reagent is a colorless transparent solution.
[0034] Reagent R2: polystyrene latex particles with a particle size of 200 nm were sensitized with an anti-human MB antibody. The reagent is a milky white solution. Specific ...
Embodiment 2
[0045] The mensuration of embodiment 2 MB
[0046] Detection tool: Hitachi 7060 automatic analyzer.
[0047] Analysis method: two-point endpoint method; main wavelength: 570nm, secondary wavelength: 800nm; sample volume: 6ul (microliter); R1: 200ul; R2: 50ul; reaction direction: rising; measurement temperature: 37°C; sample mixed with R1 After mixing, read the absorbance A at the 10th second 1 ; Add R2 at 3 to 5 minutes, and read the absorbance A after 5 minutes 2 . The absorbance of the reaction was calculated as A 2 with A 1 difference.
[0048] Calculation method: multi-point calibration, the dose / response curve is made according to the absorbance and reference serum value, and the sample content can be calculated on the dose / response curve according to its absorbance value.
Embodiment 3
[0049] Example 3 MB detection kit performance evaluation
[0050] 1. Analytical Sensitivity Assessment
[0051] Use 5% bovine serum albumin solution as a blank sample, and the blank sample should not contain the analyte. 20 consecutive detections were performed on the biochemical analyzer, and the mean and standard deviation SD of the 20 results were calculated. The detection limit of the reporting method is the mean value of the blank plus two standard deviations (+2SD). As can be seen from Table 1, the sensitivity is 1.59ug / L.
[0052] Table 1
[0053]
[0054] 2. High value linear evaluation
[0055] Mix 1 part of low-value serum (20ug / L) and 1 part of high-value serum (800ug / L) at the ratio of 9:1, 4:1, 7:3, 3:2, 1:1, 2:3, 3:7 , 1:4, 1:9 (samples numbered from 2 to 10, sample No. 1 is the low-value serum before unmixing) and mixed in proportion to prepare 10 samples with different concentrations, each sample was measured twice, from As can be seen from Table 2, th...
PUM
Property | Measurement | Unit |
---|---|---|
The average particle size | aaaaa | aaaaa |
The average particle size | aaaaa | aaaaa |
Diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
![application no application](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/application.06fe782c.png)
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com