Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Myoglobin assay kit

A technology for detecting kits and myoglobin, which is applied in the field of medical immunology, can solve the problems of increasing the amount of antibodies and production costs, false positive test results, and low accuracy, so as to improve accuracy and reliability and reduce antibody production. The effect of dosage and reduction of production cost

Active Publication Date: 2012-07-11
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] And antibody is coated on the latex, generally can adopt physical adsorption method and chemical coupling method, the sensitization latex particle stability that adopts physical adsorption method to obtain is poor, and antibody comes off easily from latex particle; , may be interfered by rheumatoid factor (RF) and heterophile antibodies, resulting in false positive or falsely elevated test results
At present, most of the chemical coupling methods used are random coupling methods, because the randomly coupled antibodies will be randomly coupled to different parts of latex organisms, which will lead to the loss of binding force of the antibodies, increasing the amount of antibodies and production costs; if the above method The obtained sensitized latex particles are used in detection kits, which will cause defects of poor specificity, low accuracy, weak anti-interference ability or high production cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Myoglobin assay kit
  • Myoglobin assay kit
  • Myoglobin assay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The present invention is further described in detail below through examples, but the present invention is not limited only to the following examples. The preparation of embodiment 1MB detection kit

[0029] The kit of the present invention relates to the main raw materials of reagents as follows:

[0030] 1. MB antibody: polyclonal antibody (commercially available).

[0031] 2. Latex: The present invention only exemplarily uses polystyrene latex particles (commercially available) with a diameter of 150-250 nm and carboxyl groups to carry out experiments.

[0032] The preparation of the main reagent of this embodiment is as follows:

[0033] Reagent R1: Glycine buffer solution containing 1.5% PEG6000 (polyethylene glycol 6000), 95 mmol / L NaCl, this reagent is a colorless transparent solution.

[0034] Reagent R2: polystyrene latex particles with a particle size of 200 nm were sensitized with an anti-human MB antibody. The reagent is a milky white solution. Specific ...

Embodiment 2

[0045] The mensuration of embodiment 2 MB

[0046] Detection tool: Hitachi 7060 automatic analyzer.

[0047] Analysis method: two-point endpoint method; main wavelength: 570nm, secondary wavelength: 800nm; sample volume: 6ul (microliter); R1: 200ul; R2: 50ul; reaction direction: rising; measurement temperature: 37°C; sample mixed with R1 After mixing, read the absorbance A at the 10th second 1 ; Add R2 at 3 to 5 minutes, and read the absorbance A after 5 minutes 2 . The absorbance of the reaction was calculated as A 2 with A 1 difference.

[0048] Calculation method: multi-point calibration, the dose / response curve is made according to the absorbance and reference serum value, and the sample content can be calculated on the dose / response curve according to its absorbance value.

Embodiment 3

[0049] Example 3 MB detection kit performance evaluation

[0050] 1. Analytical Sensitivity Assessment

[0051] Use 5% bovine serum albumin solution as a blank sample, and the blank sample should not contain the analyte. 20 consecutive detections were performed on the biochemical analyzer, and the mean and standard deviation SD of the 20 results were calculated. The detection limit of the reporting method is the mean value of the blank plus two standard deviations (+2SD). As can be seen from Table 1, the sensitivity is 1.59ug / L.

[0052] Table 1

[0053]

[0054] 2. High value linear evaluation

[0055] Mix 1 part of low-value serum (20ug / L) and 1 part of high-value serum (800ug / L) at the ratio of 9:1, 4:1, 7:3, 3:2, 1:1, 2:3, 3:7 , 1:4, 1:9 (samples numbered from 2 to 10, sample No. 1 is the low-value serum before unmixing) and mixed in proportion to prepare 10 samples with different concentrations, each sample was measured twice, from As can be seen from Table 2, th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a myoglobin assay kit. The myoglobin assay kit is characterized in that the kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is of an appropriate buffer solution; and the reagent R2 is formed by placing myoglobin antibody-sensitized polystyrene latex particles in the appropriate buffer solution. The myoglobin assay kit has the advantages of simple and fast detection, high sensitivity, good accuracy, strong anti-interference ability and low production cost.

Description

technical field [0001] The invention belongs to the field of medical immunology, and relates to an immunological detection reagent, and further, the invention relates to a myoglobin detection kit. Background technique [0002] Myoglobin (MB) is a specific oxygen-binding protein of skeletal muscle and cardiac muscle, with a molecular weight of 17.8kD. Due to its small molecular weight, when the cardiac muscle or skeletal muscle is damaged, MB will be released from the muscle tissue into the circulating blood, and can It is filtered by the glomeruli and appears in the urine. Therefore, the determination of MB in blood and urine can be used for the diagnosis of some myopathy and heart disease, such as acute myocardial infarction (AMI), acute muscle injury, acute and chronic renal failure, severe congestive heart failure, long-term shock, muscular dystrophy, diseases such as muscular dystrophy and polymyositis. [0003] AMI is a disease that seriously threatens human life. At ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
Inventor 邹炳德邹继华夏佳音
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products