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Method for promoting composite enzymes by utilizing resveratrol and oxalic acid to catalytically degrade rice straws

A technology for catalytic degradation of rice straw, applied in the field of catalytic degradation of rice straw, can solve the problems of low degradation rate of rice straw, small environmental pollution, large amount of acid and alkali, etc., and achieve the effect of improving enzymatic hydrolysis efficiency, low operating cost and low cost

Inactive Publication Date: 2014-03-19
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, many researches mainly use physical and chemical means to treat rice straw, including microwave radiation method and acid and alkali treatment method, etc., but this kind of method consumes a lot of energy, consumes a lot of acid and alkali, and has low recovery, which is easy to cause environmental pollution.
The other is the use of biotechnology to treat rice straw, the main focus of which is to use lignin degrading enzymes (such as lignin peroxidase (LiP), manganese peroxidase (MnP)) to catalyze the degradation of lignocellulose in rice straw , this kind of method has less environmental pollution and lower energy consumption, but since it is difficult for polymer substances such as LiP and MnP to enter the intact and undamaged rice straw, the degradation rate of rice straw is simply catalyzed by lignin degrading enzymes. lower

Method used

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  • Method for promoting composite enzymes by utilizing resveratrol and oxalic acid to catalytically degrade rice straws
  • Method for promoting composite enzymes by utilizing resveratrol and oxalic acid to catalytically degrade rice straws

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Embodiment 1

[0021] A kind of method utilizing veratrol and oxalic acid of the present invention to promote composite enzyme to catalyze the degradation of rice straw, comprising the following steps:

[0022] (1) Straw pretreatment: crush rice straw to obtain straw powder, pass through a 60-mesh sieve for later use;

[0023] (2) Preparation of compound enzyme solution: add 2 mL of spores to every 200 mL of sterilized liquid culture medium at a concentration of 1.5×10 6 Individual / mL Phanerochaete chrysosporium spore suspension, shake culture at 30°C and 120 r / min for 6 days, obtain compound enzyme solution (15 U / L LiP and 213 U / L of MnP); the liquid medium (1 L) formula was: glucose 10 g, ammonium tartrate 0.2 g, MgSO 4 0.71 g, Tween 80 0.5 mL, KH 2 PO 4 2.56 g, VB 1 0.01 g, sodium tartrate buffer 4.616 g, trace element solution 100 mL, benzyl alcohol 0.516 mL, H 2 O 899.484 mL, pH adjusted to 4.5;

[0024] (3) Catalytic degradation: Add the compound enzyme solution obtained in st...

Embodiment 2

[0032] A kind of method utilizing veratrol and oxalic acid of the present invention to promote composite enzyme to catalyze the degradation of rice straw, comprising the following steps:

[0033] (1) Straw pretreatment: crush rice straw to obtain straw powder, pass through a 100-mesh sieve for later use;

[0034] (2) Preparation of compound enzyme solution: Add 4 mL of spores to each 200 mL of sterilized liquid medium (the formula is the same as in Example 1) at a concentration of 2×10 6 Individual / mL Phanerochaete chrysosporium spore suspension, cultured with shaking at 37°C and 150r / min for 8 days, and obtained the compound enzyme solution (20 U / L LiP and 250 U / L of MnP);

[0035] (3) Catalytic degradation: Add the compound enzyme solution obtained in step (2) to 5 kg of rice straw powder obtained in step (1), and at the same time add a veratrol solution with a concentration of 100mM / L and an oxalic acid solution with a concentration of 800mM / L and H at a concentration of ...

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Abstract

The invention discloses a method for promoting composite enzymes by utilizing resveratrol and oxalic acid to catalytically degrade rice straws, which comprises the following steps of: (1) straw pretreatment: crushing the rice straws to obtain straw powder and screening for standby; (2) composite enzyme liquid preparation: culturing phanerochaete chrysosporium in a liquid state to obtain composite enzyme liquid; and (3) catalytic degradation: adding the composite enzyme liquid in the straw powder, simultaneously adding resveratrol solution, oxalic acid solution and H2O2 solution, heat-insulating, moisturizing and standing to finish the catalytic degradation. The method for degrading the rice straws has the beneficial effects that the corruption speed of the rice straws is accelerated, the corruption degree of the rice straws is improved, the enzymolysis efficiency of lignin is improved, the usage amount of the composite enzymes can be effectively reduced, and the industrial production can be realized; and meanwhile, the method has the advantages of low cost, simplicity in operation, low running expense, cleanness and no pollution.

Description

technical field [0001] The invention relates to the technical field of biodegradation, in particular to a method for using resatrol and oxalic acid to promote compound enzymes to catalyze the degradation of rice straw. Background technique [0002] Rice straw is an abundant renewable resource and one of the main crop straws. However, because the output of straw varies with the seasons, and the output is large, the value is low, the volume is large, and it is inconvenient to transport. The natural degradation process of straw is extremely slow, resulting in a large amount of straw being dumped directly into the environment in the form of accumulation and burning. The amount of straw is about 5×10 7 t ~ 7×10 7 t, accounting for 10% to 15% of the total straw production, causing great environmental pollution and serious waste of material resources. [0003] Straw is composed of organic matter, a small amount of minerals and water. The organic matter is mainly lignin, cellulos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/645
Inventor 黄丹莲曾茁桐曾光明赵美花赖萃危臻黄超李宁杰许飘
Owner HUNAN UNIV
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