Folic-acid-ligand-modified ferric oxide nano-particle with in vitro tumor targeting function, as well as preparation method and in vitro evaluation method thereof

Abstract

The invention discloses a folic-acid-ligand-modified ferric oxide nano-particle with an in vitro tumor targeting function, as well as a preparation method and an in vitro evaluation method thereof. The folic-acid-ligand-modified ferric oxide nano-particle with the in vitro tumor targeting function, prepared by the method disclosed by the invention, has the targeting function on in vitro tumor cells, and the mechanism of the folic-acid-ligand-modified ferric oxide nano-particle is from high expression of some malignant cells of human onto folic acid receptors. Based on detection of a test, the content of iron in human oral epidermoid cancer cells of a folic acid-dextran-ferric oxide nano-particle group highly expressed by the folic acid receptors is higher than that of iron in a dextran-ferric oxide nano-particle group which is not modified by the folic acid. Through the research on folic acid-dextran-ferric oxide nano-particles, the success rate of tumor early diagnosis is effectively improved.

Description

technical field [0001] The invention relates to a preparation method of iron oxide nanoparticles, in particular iron oxide nanoparticles modified by folic acid ligands with in vitro tumor targeting function, its preparation method and in vitro evaluation method. Background technique [0002] There are about 2.5 million new cancer patients in my country every year, and most of them have entered the middle and late stages of cancer when they are diagnosed. If the effective treatment of tumors is to be realized, it is important to realize the early diagnosis of tumors through various techniques. Therefore, the pros and cons of inspection techniques are directly related to the effect of cancer treatment. [0003] The research on tumor nanotechnology and nanomedicine has just started, and requires the use of knowledge and research methods in the fields of nanotechnology, biology, chemistry, physics, medicine, pharmacy and public health. Nanomedicine is not only an international...

AI Technical Summary

Problems solved by technology

[0007] Although many patents may have mentioned how to prepare iron ferric oxide nanoparticles, etc., the method for preparing dextran-iron oxide nanoparticles or folic acid-dextran-iron oxide nanoparticles is empirical due to the preparation process itself. Moreover, the inventors or manufacturers of each patent deliberately keep secret the details of the process, and the information provided publicly is limited, making it difficult to repeat many preparation methods and experimental schemes mentioned in many documents, or even if they can be repeated. The state of nanoparticles or their suspensions is also unstable