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High temperature resisting and high-glucose resisting lactic acid bacteria

A high-sugar, high-temperature-resistant technology applied in the field of Lactobacillus

Inactive Publication Date: 2012-07-04
天合新源(天津)能源科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Deposit date December 08, 2010

Method used

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Embodiment Construction

[0019] The following examples can enable those skilled in the art to understand the present invention more fully, but do not limit the present invention in any way.

[0020] Example 1:

[0021] The specific process is as follows:

[0022] 1. High temperature acclimatization

[0023] 1) Take a loop of Lactobacillus rhamnosus CGMCC No.1.2134 on the slant of the test tube on the ultra-clean bench, insert it into a 250mL Erlenmeyer flask containing 50mL of medium A (no agar), culture at 200rpm, 37°C for about 12h, Make the bacteria in the early logarithmic growth stage.

[0024] Medium A: (casein peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 5.0g, sodium acetate 5.0g, diamine citrate 2.0g, Tween 80 1.0g, dipotassium hydrogen phosphate 2.0g, heptahydrate Magnesium sulfate 0.2g, manganese sulfate monohydrate 0.05g, calcium carbonate 20.0g, agar 15.0g, distilled water 1.0L, pH6.8).

[0025] 2) Take 5 mL of the bacterial liquid, centrifuge at 5000 rpm for 10 minute...

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Abstract

The invention discloses high temperature resisting and high-glucose resisting lactic acid bacteria, and belongs to the field of lactic acid bacteria, in particular to high temperature resisting and high-glucose resisting strains. The lactic acid bacteria provided by the invention is Lactobacillus rhamnosus which is stored in China General Microbiological Culture Collection Center with a collection number of CGMCC No. 4430. The glucose tolerance concentration of the strain CGMCC No. 4430 reaches 270g / L which is improved by 95% compared to the glucose tolerance concentration of the primary strain. After fermentation, the content of lactic acid is 50g / L which is increased by 158% compared to the concentration of the primary strain. The performance indicators followed in fermentation tank experiment show that the strain which is normal in metabolism and has strong L-lactic acid-producing capacity and low content of heteroacid is a high temperature resisting and high-glucose resisting Lactobacillus rhamnosus strain.

Description

Technical field: [0001] The invention belongs to the field of lactobacilli, in particular to high-temperature-resistant and high-sugar-resistant lactobacillus strains. Background technique: [0002] In recent years, due to some special functions, the research of extremophiles has attracted more and more attention. Among them, high temperature resistant and hypertonic lactic acid bacteria are particularly prominent due to their wide application in various industries such as food, medicine and chemical industry. At present, its main applications include the following aspects: (1) Lactic acid: Lactic acid is an important food and chemical raw material, and can be used to prepare a degradable polymer material - polylactic acid, so it has attracted much attention in recent years. (2) Biological preservatives: Nisin is a biological preservative widely used in food and beverages in recent years. Compared with chemical preservatives, it is harmless to the human body and has a wider...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12P7/56C12R1/225
Inventor 李政张健飞王玉李绩
Owner 天合新源(天津)能源科技有限公司
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