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Antibacterial latex contraceptive material provided with grafted nanoscale IFN (Interferon)-alpha

A nano-scale, latex technology, applied in male contraceptives and other directions, can solve the problem of high cost, achieve the effect of data system and rigorous, high antibacterial performance, strong antibacterial effect

Inactive Publication Date: 2012-07-04
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the tensile strength of the polyurethane material is twice that of the rubber film, after testing, the OST laboratory report and related research results show that its slippage and rupture rate, bacterial penetration rate, contraceptive and sexually transmitted disease shielding effects are all comparable to rubber condoms Similar and costly, it is mainly used for female condoms (Tang Wenhao, Gu Yiqun, Chen Zhenwen. Review and prospect of research on male birth control methods. Chinese Journal of Family Planning. 2002; 10(5): 312-314; Nicholas D. W, David M. H, Steffen B. Male condoms that break in use do so mostly by a “blunt puncture” mechanism. Contraception. 2008; 77(5): 360-365; Mags B, Jenni S, Zonke M, et al . Performance of the Reality? polyurethane female condom and a synthetic latex prototype: a randomized crossover trial among South African women. Contraception. 2006; 73(4): 386-393)

Method used

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  • Antibacterial latex contraceptive material provided with grafted nanoscale IFN (Interferon)-alpha
  • Antibacterial latex contraceptive material provided with grafted nanoscale IFN (Interferon)-alpha
  • Antibacterial latex contraceptive material provided with grafted nanoscale IFN (Interferon)-alpha

Examples

Experimental program
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Embodiment 1

[0032] Example 1 Preparation of antibacterial latex contraceptive material grafted with nanoscale IFN-α

[0033] 1. Preparation and detection of photoactive IFN-α

[0034] Add IFN-α (30μg, 0.002μmol) to 5ml containing N -Succinimide (23.04 μg, 0.14 μmol) in dimethylamide DMF / PBS (pH=7.4, volume ratio 4:1) solution, stirred and reacted at 4°C (ice bath) for 48h. After the synthesis, use an ultrafiltration membrane (Milipore Molecut II, 10k Na) to purify the azidophenyl-derived IFN-α by intermittent centrifugation at 4000rpm for 4h, add PBS(-) solution to dissolve, and adjust to a certain concentration , and kept frozen for later use. Since the azide group has a characteristic peak of light absorption, we analyze the sample by IR, UV and XPF.

[0035] IR / UV / XPF spectra were drawn and analyzed by Origin Pro 7.5, FESEM images were analyzed by Image pro plus 6.0, and AFM (Bioscope Catalyst, Veeco, USA) was analyzed by Nanoscope Analysis software.

[0036] The infrared spectru...

Embodiment 2

[0049] Example 2 Antibacterial experiment of antibacterial latex contraceptive material grafted with nanoscale IFN-α

[0050] Neisseria gonorrhoeae, one of the main pathogens of cervicitis, can be cultured in a nutrient-rich medium and a suitable environment, and grow into colonies with certain characteristics. Typical morphology of Neisseria gonorrhoeae colonies are: round, small, raised, smooth, moist, translucent or off-white colonies. The edge of the colony is petal-shaped, with a diameter of 0.5-1mm, easy to emulsify, and sticky. In this embodiment, the grafted surface of the material is covered on the TM medium to act on Neisseria gonorrhoeae, and after a period of time, the colonies of Neisseria gonorrhoeae on the surface of the material are observed. For the flow chart of this experiment, see Figure 5 .

[0051] 1. Diffusion method test

[0052] Refer to the representative evaluation method of Japanese antibacterial agents, learn from the bacterial inspection me...

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Abstract

The invention discloses an antibacterial latex contraceptive material provided with grafted nanoscale IFN (Interferon)-alpha, which is formed by grafting and fixing the IFN-alpha onto the surface of a latex contraceptive material. The invention also discloses a preparation method of the material, which adopts a photochemical fixing method and specifically comprises the following steps of: adding the IFN-alpha into dimethyl amide solution containing N-succinimide, agitating and reacting for 48 hours at a temperature of 4 DEG C, synthesizing the IFN-alpha with the photoactivity, smearing the IFN-alpha with the photoactivity onto the surface of the latex contraceptive material and carrying out ultraviolet radiation after shading, freezing and drying. The antibacterial latex contraceptive material provided with the grafted nanoscale IFN-alpha has the beneficial effects that IFN-alpha particles belong to nanoscale and are uniformly arranged on a latex film; the antibacterial effect is remarkably superior to that of a latex material on which the IFN-alpha is directly smeared; and the latex material provided with the grafted the IFN-alpha not only can be taken as the contraceptive material, but also can be made into other gynecological supplies and can be matched with traditional drugs to commonly prevent, even treat the inflammation of cervix.

Description

technical field [0001] The invention relates to a bacteriostatic contraceptive material, in particular to a bacteriostatic latex contraceptive material grafted with nanometer-level IFN-α. Background technique [0002] Cervicitis (Inflammation of Cervix) is the most common inflammatory disease of the female reproductive system, with bacterial vaginal pathogen infection as the main cause. coccus is the main causative agent of mucopurulent cervicitis (Sarabeth B, Jennifer L, Devon J, et al . Cervicitis in Adolescents: Do We Know Correct Diagnosis and Treatment Guidelines? Journal of Pediatric and Adolescent Gynecology, 2010; 23(2): 67), and also closely related to high-risk human papillomavirus (HPV) infection (Harald zur Hausen . HPV Today [Z / OL]. Newsletter on Human Papillomavirus. 2009), it is known that more than 90% of cervical cancer patients are associated with high-risk HPV infection (LE J. Gynecology and Obstetrics. 7th ed. Beijing: People's Medical Publishing House, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08J3/28C08L9/10A61F6/04
Inventor 关燕清刘俊明张琳
Owner SOUTH CHINA NORMAL UNIVERSITY
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