Microporous plate and kit for food allergen IgG antibody detection, preparation methods thereof, and detection method

A technology for food allergy and antibody detection, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of time-consuming, labor-intensive, expensive, and the cause of disease cannot be found, and achieve considerable economic benefits, high costs, and good social benefits.

Inactive Publication Date: 2012-06-27
北京北方北方有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Symptoms or diseases like these often have a serious impact on the patient's work and life. If they receive drug treatment for this, not only will they not be able to eradicate the root cause of the disease, but may also bring heavy economic burdens to the patient and their families
[0005] Due to the wide variety of food allergens, there are more than ten common and representative types. The existing methods for detecting IgG antibodies to food allergens are mainly ELISA methods, but they are all single-index detection. A sample requires multiple Simultaneous detection of kits is expensive, time-consuming and laborious, and often the cause cannot be found due to incomplete index detection, which is difficult to meet clinical needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of 14 kinds of food allergen IgG antibody detection microwell plates and kits

[0039] 1. Dissolve 14 food allergen proteins (beef, chicken, cod, corn, crab, egg white / yolk, mushroom, milk, pork, rice, shrimp, soybean, tomato, wheat) in 50mM carbonic acid buffer at 10μg / ml solution, mix well.

[0040] 2. Put them into the microwell plate in a certain order (100 μl per well), incubate overnight at 4°C, wash the plate with Tween-containing phosphate buffer for 3 times, and then add blocking solution (containing 2 %BSA in phosphate buffer), 200 μl per well, and incubated overnight at 4°C.

[0041] 3. Discard the liquid in the well, dry the microporous plate overnight at room temperature, and seal the microporous plate in an aluminum foil bag after drying.

[0042] 4. Weigh 10g of BSA and dissolve it in 1 liter of 50mM phosphate buffer solution to prepare sample diluent, and pack in 50ml bottles.

[0043] 5. Horseradish peroxidase and anti-human Ig...

Embodiment 2

[0052] Embodiment 2: the detection method of food allergen IgG antibody

[0053] Equilibrate all reagents to room temperature before use.

[0054] 1. Prepare to draw a standard curve:

[0055] Take four 12x75mm glass tubes and mark 50, 100, 200 and 400U / ml respectively. Add 150 μl of serum dilution to these 4 tubes sequentially. Add 150 μl of 14 kinds of food allergen IgG antibody standard serum to the tube marked 400U / ml. After mixing, take 150μl and add it to the tube marked 200U / ml; after mixing again, take 150μl and add it to the tube marked 100U / ml; after mixing the same, take 150μl and add it to the tube marked 50U / ml. In this way, 150 μl of IgG antibody standard serum with a concentration of 100, 200 and 400 U / ml, respectively, and 300 μl of IgG antibody standard serum with a concentration of 50 U / ml were obtained. Take 100 μl from each tube and add it into the microwell plate: draw the standard curve with the concentration as the abscissa and the absorbance as the ...

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PUM

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Abstract

The invention discloses a microporous plate for the food allergen IgG antibody detection. The microporous plate is coated with various food allergen proteins. The invention also discloses a kit for the food allergen IgG antibody detection and containing the microporous plate, preparation methods of the microporous plate and the kit, and a detection method. According to the invention, detections of various indexes (with the number of about 2-90) can be simultaneously carried on one microporous plate, and the quantitative grading can be realized, so strong bases are provided for a doctor to determine the severe degree of a disease and guide the treatment, and problems of time and labor consuming and high cost of detections of single indexes are simultaneously solved.

Description

technical field [0001] The invention relates to the field of ELISA detection, in particular to a microporous plate for detection of food allergen IgG antibodies, a kit, a preparation method and a detection method. Background technique [0002] With the development of society and the advancement of science and technology, people's pace of life is getting faster and faster, and the types of food are becoming more and more complicated. Subsequently, many symptoms such as irritable bowel syndrome, chronic fatigue syndrome, and insomnia also plagued more and more people, more and more people suffered from chronic diseases, and more people In a sub-healthy state. However, when these people go to the hospital for testing, they often can't find out the symptoms, and can't clarify the cause and take corresponding treatment measures. In developed countries such as Europe and the United States, the detection technology for this group of people has developed significantly, and enzyme-...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/531
Inventor 刘兴旺程晓雷叶兴旺
Owner 北京北方北方有限责任公司
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