Culture medium for improving germination and growth of Luntai white apricot blossom pollen
A technology for pollen germination and culture medium, which is applied to plant cells and other directions, can solve the problems of low pollen germination rate, difficult pollination and fertilization, short pollen tubes, etc., and achieves the effects of promoting pollen germination, convenient purchase and low price.
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[0033] All reagents and equipment used are well known in the art.
[0034] Selection and processing of pollen:
[0035] Select flower buds at the bell stage, peel off the anthers and store them in a sulfuric acid carton, place them in the shade at room temperature, wait for the anthers to split and release pollen, collect them into a 1.5ml centrifuge tube, seal them, and store them in the freezer of the refrigerator;
[0036] Cultivation method and time:
[0037]Medium configuration: use 1% agar, respectively put in the concentration: 0, 5%, 10%, 15%, 20% or 25% of sucrose and the concentration: 0, 0.01%, 0.03%, 0.05% or 0.1 % boric acid treatment, put different concentrations of culture medium upside down on the glass slide, use cotton swabs to evenly pop the pollen on the glass slide culture medium, put it in a petri dish with wet gauze at the bottom, repeat 3 times for each gradient The second time, put them in a 25°C incubator for cultivation, and observe the germination...
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