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High-selenium liquid culture method for pleurotus eryngii

A technology of liquid culture and king oyster mushroom, applied in the directions of botanical equipment and methods, horticulture, application, etc., to achieve good effects

Inactive Publication Date: 2012-06-27
SUZHOU SETEK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no research and development has been carried out on the cultivation of Pleurotus eryngii in liquid selenium-enriched materials to obtain high-selenium materials, as additives for food and health products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: 3L liquid submerged fermentation

[0057] 1) Purchase Pleurotus eryngii strains (No. 5.775) from the Culture Collection Center of the Chinese Academy of Sciences.

[0058] 2) Insert Pleurotus eryngii strains into the sterilized PDA comprehensive medium for in vitro strain culture.

[0059] 3) sodium selenite (Na 2 SeO 3 ) and the PDA comprehensive culture medium are mixed according to a certain ratio, and the selenium content is 10, 30, 50, 60, 80, 100, 120mg / L, and the selenium-containing medium is sterilized for subsequent use.

[0060] 4) Insert the test-tube strain cultivated in step 2 into the culture medium with a selenium concentration of 10 mg / L prepared in step 3, and carry out primary selenium-tolerant cultivation of Pleurotus eryngii strain.

[0061] 5) Insert the primary selenium-tolerant Pleurotus eryngii strain cultivated in step 4 into the 30 mg / L medium prepared in step 3, and carry out secondary selenium-tolerant cultivation of the pleu...

Embodiment 2

[0071] Embodiment 2: 10L liquid submerged fermentation

[0072] 1) Purchase Pleurotus eryngii strains (No. 5.775) from the Culture Collection Center of the Chinese Academy of Sciences.

[0073] 2) Insert Pleurotus eryngii strains into the sterilized PDA comprehensive medium for in vitro strain culture.

[0074] 3) sodium selenite (Na 2 SeO 3 ) and the PDA comprehensive culture medium are mixed according to a certain ratio, and the selenium content is 10, 30, 50, 60, 80, 100, 120mg / L, and the selenium-containing medium is sterilized for subsequent use.

[0075] 4) Insert the test-tube strain cultivated in step 2 into the culture medium with a selenium concentration of 10 mg / L prepared in step 3, and carry out primary selenium-tolerant cultivation of Pleurotus eryngii strain.

[0076] 5) Insert the primary selenium-tolerant Pleurotus eryngii strain cultivated in step 4 into the 30 mg / L medium prepared in step 3, and carry out secondary selenium-tolerant cultivation of the ple...

Embodiment 3

[0086] Embodiment 3: 30L liquid submerged fermentation

[0087] 1) Purchase Pleurotus eryngii strains (No. 5.775) from the Culture Collection Center of the Chinese Academy of Sciences.

[0088] 2) Insert Pleurotus eryngii strains into the sterilized PDA comprehensive medium for in vitro strain culture.

[0089] 3) sodium selenite (Na 2 SeO 3 ) and the PDA comprehensive culture medium are mixed according to a certain ratio, and the selenium content is 10, 30, 50, 60, 80, 100, 120mg / L, and the selenium-containing medium is sterilized for subsequent use.

[0090] 4) Insert the test-tube strain cultivated in step 2 into the culture medium with a selenium concentration of 10 mg / L prepared in step 3, and carry out primary selenium-tolerant cultivation of Pleurotus eryngii strain.

[0091] 5) Insert the primary selenium-tolerant Pleurotus eryngii strain cultivated in step 4 into the 30 mg / L medium prepared in step 3, and carry out secondary selenium-tolerant cultivation of the ple...

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PUM

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Abstract

The invention discloses a high-selenium liquid culture method for pleurotus eryngii. The method comprises the following steps of: (1) inoculating pleurotus eryngii strains into a PDA (Potato Dextrose Agar) comprehensive culture medium to perform test tube strain culture to obtain initial flora suspension; (2) mixing a selenium source and the PDA comprehensive culture medium to prepare selenium-containing culture mediums with gradient selenium concentrations respectively; sterilizing the selenium-containing culture mediums; inoculating the pleurotus eryngii strains into the sterilized selenium-containing culture mediums in a flora suspension inoculation mode to perform selenium-tolerant culture at the selenium concentrations one by one in the conventional pleurotus eryngii strain culture mode in an order from low to high of the selenium concentration, wherein the flora suspension used in the primary selenium-tolerant culture is initial flora suspension, the flora suspension adopted by the subsequent selenium-tolerant culture is the flora suspension used for performing the selenium-tolerant culture at the current selenium concentration, and the like; and the best selenium tolerance concentration of the pleurotus eryngii and the highly selenium-tolerant pleurotus eryngii strains are obtained; and (3) performing fermentative culture on the highly selenium-tolerant pleurotus eryngii strains obtained by the step (2). After the highly selenium-tolerant culture of the pleurotus eryngii strains, the tolerance against selenium content in a culture medium is greatly improved in the state of liquid submerged culture.

Description

technical field [0001] The invention relates to a preparation method of a high-selenium material, in particular to a high-selenium liquid culture method of Pleurotus eryngii. Background technique [0002] Selenium is an essential trace element in humans and animals. It is the active center that constitutes as many as 23 functional proteins such as glutathione peroxidase (GSH-Px) and selenoprotein P. It has the following functions: (1) remove Peroxides and free radicals can protect cell structure and function from interference and damage, and delay aging; (2) accelerate the metabolism of carcinogens, promote DNA damage repair, and reduce the incidence of cancer; (3) antagonize toxic substances in the body and reduce their (4) Enhance the body's cellular immune function and improve the body's immunity. However, about 72% of the population in my country is in a state of selenium deficiency, and the total daily selenium intake is about 35 μg, which is significantly lower than t...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05D9/02
Inventor 袁林喜胡海涛
Owner SUZHOU SETEK
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