Culture medium for indoor cultivation for selenium enrichment stropharia rugoso-annulata and cultivating method
A culture medium and a technology for the cultivation of the giant globulus, which is applied to the medium and the cultivation field for indoor cultivation of the selenium-enriched globular mushroom, and can solve the problems of being unsuitable for the growth and high yield of the globular mushroom, the complex production process of the strain, and the raw materials. There are many varieties and other problems, to achieve the effect of alleviating heavy metal toxicity, reducing heavy metal toxicity, and strong anti-cancer activity
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Embodiment 1
[0036] A culture medium for indoor cultivation of selenium-enriched Stropharia stropharia comprises a mother seed medium, an original seed medium, a cultivar medium and a stropharia stropharia cultivation material.
[0037] The mother seed culture medium includes the following raw materials: 45g of bran, 180g of potato, 15g of glucose, 0.5g of potassium dihydrogen phosphate, 0.05g of sodium selenite, 0.5g of magnesium sulfate, 15g of agar, and 800g of water.
[0038] The preparation method of the mother seed medium is as follows: (1) Wrap the bran with gauze, put it into a stainless steel pot, add water, boil the water and keep it for 10 minutes; , potatoes are fished out, and agar is added to the stainless steel pot water, and glucose, potassium dihydrogen phosphate, sodium selenite, magnesium sulfate are put into after the agar melts; (3) adjust the pH value to 6, filter, and obtain the obtained Put the mother culture medium into the test tube, cover it with a silica gel plu...
Embodiment 2
[0053] A culture medium for indoor cultivation of selenium-enriched Stropharia stropharia comprises a mother seed medium, an original seed medium, a cultivar medium and a stropharia stropharia cultivation material.
[0054] The mother seed medium includes the following raw materials: 55g of bran, 220g of potato, 25g of glucose, 1.0g of potassium dihydrogen phosphate, 0.15g of sodium selenite, 1.0g of magnesium sulfate, 25g of agar, and 1200g of water.
[0055] The preparation method of the mother seed medium is as follows: (1) Wrap the bran with gauze, put it into a stainless steel pot, add water, boil the water and keep it for 10 minutes; , Potatoes are fished out, add agar to stainless steel pot water, put into glucose, potassium dihydrogen phosphate, sodium selenite, magnesium sulfate after the agar melts; (3) adjust the pH value to 7, filter, and obtain Put the mother culture medium into a test tube, place it vertically in an autoclave, sterilize it under a high pressure o...
Embodiment 3
[0070] A culture medium for indoor cultivation of selenium-enriched Stropharia stropharia comprises a mother seed medium, an original seed medium, a cultivar medium and a stropharia stropharia cultivation material.
[0071] The mother seed medium includes the following raw materials: 50 g of bran, 200 g of potato, 20 g of glucose, 0.5 g of potassium dihydrogen phosphate, 0.1 g of sodium selenite, 0.5 g of magnesium sulfate, 20 g of agar, and 1000 g of water.
[0072] The preparation method of the mother seed medium is as follows: (1) Wrap the bran with gauze, put it into a stainless steel pot, add water, boil the water and keep it for 10 minutes; , potatoes are fished out, and agar is added to the stainless steel pot water, and glucose, potassium dihydrogen phosphate, sodium selenite, magnesium sulfate are put into after the agar is melted; (3) adjust the pH value to 6.5, filter, and obtain final product Put the mother culture medium into a test tube, place it vertically in an...
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