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Method for breeding new line of Brassica napus by utilizing biotechnology

A biotechnology and new line technology, which is applied in the genetic field of yellow seed traits of local variety mustard type yellow seed rape, can solve the problems of genetic instability, difficulty in utilization and low efficiency of yellow seed traits, shorten the breeding time, The effect of reducing workload

Inactive Publication Date: 2012-06-20
NORTHWEST A & F UNIV
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Problems solved by technology

[0006] 1. In Brassica napus, the genetic basis of yellow seed germplasm is narrow, and the inheritance of yellow seed traits is unstable, making it difficult to utilize
[0007] 2. The selection efficiency of the traditional backcross transfer method is low, it takes a long time and it is difficult to effectively improve the yellow seed traits of Brassica napus
[0008] 3. The established genetic linkage map of yellow seed traits is relatively rough, and it is difficult to use in yellow seed gene cloning and yellow seed breeding

Method used

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  • Method for breeding new line of Brassica napus by utilizing biotechnology
  • Method for breeding new line of Brassica napus by utilizing biotechnology
  • Method for breeding new line of Brassica napus by utilizing biotechnology

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Embodiment Construction

[0019] The method of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0020] refer to figure 1 , figure 2 , the present invention utilizes Shaanxi yellow mustard (pure yellow seed) and Guanzhong mustard (brown seed) to hybridize, and its F 1 Generation selfing to get an F 2 group, each F 2 Generation single plant selfing, judge F according to the color of the kernels on the selfing single plant 2 Phenotype of the plant. The combination of AFLP, SSR technology and group analysis (BSA) method was used to screen the molecular markers closely linked to brown seed genes, and transform these markers into more stable and easy-to-operate SCAR markers. The results showed that there were 21 AFLP markers and 2 SSR markers closely linked with brownseed gene, among which the SSR marker CB10022 was a co-dominant marker. The nearest molecular markers on both sides of the yellow seed locus were EA0...

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Abstract

The invention provides a method for breeding a new line of Brassica napus by utilizing a biotechnology. The method comprises the steps of: selecting each individual from separation colonies BC1F1, BC2F1, BC3F1, BC4F1 and BC5F1 utilizing a codominant mark CB10022, selecting individuals with Bb genotype and eliminating non-target individuals in combination with field inspection; at the same time, carrying out background selection on the target individuals by utilizing AFLP primers, carrying out quality analysis on the target individuals in each separation colony to select double-low offspring, finally selecting three individuals with highest similarity with recurrent parent Xu102 and carrying out backcrossing; and carrying out foreground selection in BC5F2 by utilizing SSR mark CB10373 and SCAR mark to select individuals with bb genotype, and then carrying out selfing on each generation to obtain the stable Brassica napus line. By adopting the method, the breeding time of the Brassica napus line is greatly shortened and the workload is reduced.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and relates to the research on creation of Brassica type yellow-seeded rapeseed by interspecific hybridization, and specifically relates to the inheritance of yellow-seeded traits of local varieties of mustard-type yellow-seeded rapeseed, molecular markers and the use of molecular markers to select and breed new strains of yellow-seeded rapeseed method. Background technique [0002] Compared with black seed rape, yellow seed rape has the advantages of high oil content, high protein content, thin seed coat, low fiber content, clear and transparent oil, and good quality. Since Professor Liu Houli first discovered that the oil content of yellow-seed rape was higher than that of non-yellow-seed rape in China in the 1960s, the research and utilization of rapeseed yellow-seed genes has always been a hot spot in the field of rape research. Many studies have proved that yellow rapeseed can increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/68
Inventor 黄镇徐爱遐班元元张学贤
Owner NORTHWEST A & F UNIV
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