Technical method for rapid asexual propagation and anti-enhancement of hybrid bollgard F1

Abstract

The invention relates to a technical method for rapid asexual propagation and anti-enhancement of hybrid bollgard F1, which comprises the main steps of: firstly, identifying perennial cotton germplasm resources capable of naturally overwintering, and resisting fusarium wilt resistance and greensickness; adopting the perennial cotton germplasm resources as rootstock, scioning and grafting with the hybrid bollgard F1, and cultivating perennial root to obtain grafted plants; and secondly, carrying out large-scale propagation of hybrid bollgard F1 seedlings by using the method, and directly using the hybrid bollgard F1 seeldings for agricultural production. According to the technical method for rapid asexual propagation and anti-enhancement of the hybrid bollgard F1, field cotton hybrid seed production is free, the seed production process of the hybrid bollgard F1 is simplified, the seed purity of the hybrid bollgard F1 is improved, and the disease resistance of the hybrid bollgard F1 is enhanced. The method can be used for large-scale propagation of hybrid bollgard F1 seedlings with obviously improved anti-enhancement property, and is applied to agricultural production.

Description

technical field [0001] The invention relates to the field of popularization and application of hybrid insect-resistant cotton F1, in particular to a technical method for asexual rapid propagation and resistance enhancement of hybrid insect-resistant cotton F1. Background technique [0002] At present, the main types of cotton cultivated are conventional cotton and hybrid cotton. 70% of the cotton area in the Yangtze River Basin is planted with F1, an insect-resistant hybrid cotton that is crossed between varieties. The production of hybrid cotton seeds among existing varieties all adopts artificial detasseling, artificial pollination and hybridization to obtain hybrid F1 seeds. Its main disadvantages are: [0003] (1) For the production of hybrid cotton seeds, parental breeding and hybrid seed production must be carried out separately, and impurities and impurities must be removed every year. [0004] (2) Disorderly competition in seed production bases makes it difficult...

AI Technical Summary

Problems solved by technology

[0004] (2) Disorderly competition in seed production bases makes it difficult for enterprises to guarantee the management and technical level of the bases, which directly affects the quality of cotton seeds

[0005] (3) Since manual detasseling and pollination requires a large amount of concentrated labor, under the current situation of few agricultural workers, high wages, and relatively low efficiency in seed production, the production of hybrid cotton seeds is stuck in a bottleneck, and it is difficult to expand the scale, and it is difficult to guarantee the quality of the seeds

[0007] (1) Cotton tissue culture is limited by the genotype of embryogenesis and plant regeneration;

[0008] (2) The cultivation cycle is long and the process is complicated, mostly at the level of obtaining a small number of plants through indirect, long-term and complicated multiple subcultures;

[0009] (3) The experiment is unstable and the repeatability is poor. low regeneration rate;

[0010] (4) The deformity of cotton seedlings obtained from cotton tissue culture is very serious;

[0011] (5) Lack of a complete cotton tissue culture and efficient plant regeneration system suitable for a wide range of genotypes;

[0012] (6) The transplanting survival rate of regenerated plants is low