Method for creating common maize inbred-line by introducing teosintes in maize cultivated specie

Abstract

The invention discloses a method for creating a common maize inbred-line by introducing teosintes in a maize cultivated specie, belonging to a method for creating maize breeding materials in crop breeding. The method integrates multiple technologies of creating the common maize inbred line, comprising the following steps: step 1, environment variation: giving only 12 hours of illumination everyday in an artificial darkroom to let teosintes and the maize cultivated specie flower in the same period; step 2, introduction of hybridization: according to a routine hybridization bagging method, using the cultivated specie as the female parent and the teosintes as the male parent to hybridize; step 3, backcrossing improvement: using the female parent cultivated specie corresponding to F1 as the female parent, taking F1 mixed pollen to backcross with the female parent to obtain BC1 seeds, sowing the BC1 seeds, taking BC1 mixed pollen to carry out secondary backcross with the female parent to obtain backcrossing second generation (BC2) seeds; step 4, pedigree breeding: sowing the BC2 seeds, preferably individual plants, carrying out continuous inbreeding of more generations to obtain a stable inbred-line; and step 5, using the stable inbred-line as the female parent, taking test line pollen to hybridize, planting and identifying the hybridized combinations, and comparing and screening excellent combinations.

Description

technical field [0001] "Method of introducing teosinte into maize cultivars to create common maize inbred lines" belongs to agronomy (code: 210) - agronomy (code: 210.30) - crop breeding and improved seed breeding (code: 210.3035) in maize Breeding material creation method. Background technique [0002] Corn is one of the three major grain crops (rice, wheat, corn) in China, with a perennial cultivated area of ​​nearly 400 million mu, and its total and per unit yield ranks second among grain crops; corn is also the main livestock feed and important food , medicine, and industrial raw materials, therefore, corn plays a pivotal role in my country's national economic and social development. In the past 30 years, the level of corn technology in my country has been significantly improved, and the yield per unit area and total yield have nearly doubled, among which the genetic gain due to variety improvement has played a leading role. [0003] The characteristics of maize variety...

AI Technical Summary

Problems solved by technology

Therefore, artificial environment induction must be carried out

Images