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Preparation method of promoter for producing ceramide and/or glucosylceramide

A technology of ceramide and manufacturing method, applied in the field of ceramide and/or glucosylceramide production accelerator, to achieve the effect of improving moisturizing ability

Inactive Publication Date: 2012-05-30
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is no report on the use of microorganisms of the genus Debaryomyces to produce ceramide and / or glucosylceramide production promoters

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (Manufacture of ceramide and / or glucosylceramide production promoter)

[0051] Debaryomyces hansenii (purchased from Jiangnan University and preserved in CICIM, China Universities Industrial Microbiology Resources and Information Center, original No. 476-20-1).

[0052] Inoculate the above strain obtained by culturing in YPD (Yeast Extract Peptone Dextrose Medium) medium at 30°C into a Erlenmeyer flask (capacity 300ml) containing 100ml of ceramide / glucosylceramide production promoter production medium, and inoculate at 30°C cultured for 72 hours. The medium contained 10 w / v% okara and 1 w / v% glucose. Wherein, the preparation of soybean dregs is obtained by soaking soybeans in water for 12 hours to make soybeans fully absorb water, then grinding and crushing the soybeans that have absorbed water, filtering and removing liquid components, and draining remaining residues.

[0053] Then, in order to recover the ceramide and / or glucosylceramide production accelerator from...

Embodiment 2

[0056] Debaryomyces hansenii (purchased from Jiangnan University and preserved in CICIM, the Industrial Microbiology Resource and Information Center of Chinese Universities, original number 514) was used as a microorganism for producing ceramide / glucosylceramide production accelerator from bean dregs. -20-4). The same method as in Example 1 was used to obtain the soybean dregs fermented extract as the ceramide / glucosylceramide production accelerator of the present invention.

[0057] Furthermore, the okara fermented extract was dissolved in 50v / v% ethanol, adjusted to 1w / v% concentration of the okara fermented extract, stored at 4°C, and used in the subsequent ceramide / glucoceramide production promotion test.

Embodiment 3

[0059] Debaryomyces hansenii (purchased from Jiangnan University and deposited in CICIM, China University Industrial Microbiology Resources and Information Center, accession number CICIMY0356) was used as a microorganism for producing ceramide / glucosylceramide production accelerator from bean dregs. ). The same method as in Example 1 was used to obtain the soybean dregs fermented extract as the ceramide / glucosylceramide production accelerator of the present invention.

[0060] Furthermore, the okara fermented extract was dissolved in 50v / v% ethanol, adjusted to 1w / v% concentration of the okara fermented extract, stored at 4°C, and used in the subsequent ceramide / glucoceramide production promotion test.

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PUM

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Abstract

The invention provides a preparation method of a promoter for producing ceramide and / or glucosylceramide, wherein debaryomyces microbes are cultured in a culture medium containing bean dregs and saccharides, and the promoter for producing ceramide and / or glucosylceramide is obtained from the supernatant. According to the preparation method of the invention, special microbes are used, and bean dregs which are by products in food processing industry are used as a main raw material of the culture medium; therefore the promoter for producing ceramide and / or glucosylceramide is obtained with low cost and high efficiency.

Description

technical field [0001] The present invention relates to a method for producing a ceramide and / or glucosylceramide production promoter capable of promoting the production of ceramide and / or glucosylceramide in the skin by using specific microorganisms and using bean dregs, a by-product of the food processing industry, etc., as the main raw material of a culture medium . Background technique [0002] Ceramide, one of the sphingolipids, exists in the stratum corneum of the skin and accounts for about 50% of the intercellular lipids in the stratum corneum. Sphingolipid is a general term for complex lipids having a sphingosine skeleton. Glucosylceramide is a kind of sphingolipid formed by binding glucose to ceramide. The ceramide synthesized by epidermal cells is temporarily stored in the form of glucosylceramide or sphingomyelin; it is then excreted outside the cell, where glucocerebrosidase (Glucocerebrosidase) and sphingomyelinase (Sphingomyelinase) Under the action of cera...

Claims

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Application Information

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IPC IPC(8): C12P1/02A61K8/99A61Q19/00C12R1/645
Inventor 孔凡旗
Owner KAO CORP
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