Method for combined preparation of high purity ursolic acid and high purity oleanolic acid from Ligustrum lucidum

A technology for oleanolic acid and combined preparation is applied in the field of natural medicines and achieves the effects of easy handling, low cost and simple method.

Inactive Publication Date: 2012-05-23
曾超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reported extraction method is mainly based on organic solution leaching in combination with certain separation means. For example, CN1358733 obtains ursolic acid in Ligustrum privet leaves by combining methods such as ethanol reflux extraction, activated carbon decolorization, drying, etc., although this invention is more convenient, But only crude ursolic acid can be obtained

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Take 1Kg of dried privet leaves, add 8L of 5% sodium hydroxide aqueous solution, boil for 30 minutes, filter out the alkaline solution while hot, wash the medicinal residue with hot water, add the residue to 10L of water and boil for 1.5 hours, pour off the boiling liquid, The residue was refluxed and extracted with 95% ethanol for 3 times, and the amount of ethanol was 6L each time; the alcoholic extract was combined, and 0.3% activated carbon was added to the alcoholic extract, and stirred at 50-60°C for 1 hour for decolorization; The extract was adjusted to a pH of about 11 with 1mol / L sodium hydroxide aqueous solution, and filtered after standing for 12 hours to remove the precipitate, and the filtrate was concentrated under reduced pressure to about 1 / 3 (5L) of the volume before concentration; a large amount of insoluble matter was precipitated , this insoluble matter is the crude product of oleanolic acid, filtered, and the filtrate is adjusted to pH between 3-4 wi...

Embodiment 2

[0017] Take 1Kg of dried Ligustrum lucidum, add 8L of 5% sodium hydroxide aqueous solution, boil for 30 minutes, filter out the alkaline solution while hot, wash the medicinal residue with hot water, add the residue to 10L of water and boil for 2 hours, pour off the water and boil solution, the residue was refluxed with 90% ethanol for 3 times and the amount of ethanol was 6L each time; the alcohol extract was combined, and 0.3% of the volume of the alcohol extract was added with activated carbon, and stirred and decolorized at 50-60°C for 1 hour; the activated carbon was filtered off, The decolorized alcoholic extract is adjusted to a pH of about 11 with 1mol / L sodium hydroxide aqueous solution, filtered after standing for 12 hours, and the precipitate is removed, and the filtrate is concentrated under reduced pressure to about 1 / 3 (5L) of the volume before concentration; there is a large amount of insoluble The insoluble matter is the crude product of oleanolic acid, which is...

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Abstract

The invention which discloses a method for the combined preparation of high purity ursolic acid and high purity oleanolic acid from Ligustrum lucidum belongs to the technical field of natural medicines, wherein the method comprises processes for extracting and purifying ursolic acid and oleanolic acid from Ligustrum lucidum leaves or Ligustrum lucidum seeds. The method adopted in the invention allows the cheap and easily available Ligustrum lucidum leaves or Ligustrum lucidum seeds which are treated as a raw material to be extracted by refluxing with ethanol. The treatment for "three wastes" has the advantages of small capacity and easiness, ethanol can be recycled, and waste residue residual after the extraction can be used as a fertilizer, so the method basically generates no pollution to environment, and the cost of the obtained ursolic acid is low. In addition, the method of the invention is simple and fast. The obtained ursolic acid is a raw material of medicines for blood fat reduction and cardiovascular disease treatment; and the obtained oleanolic acid is an auxiliary hepatopathy medicine recorded by a nonprescription medicine directory in China. The method is suitable for the industrialization large-scale production.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, and in particular relates to a method for extracting and purifying its main active components, ursolic acid and oleanolic acid, from privet privet. technical background [0002] Ligustrum is a plant of the genus Ligustrum of the Oleaceae family. The scientific name is Ligustrum Lucidum Ait. It is a large evergreen shrub or small tree in all seasons. There are about 50 species in the world, and there are 38 species in my country. They are widely distributed in East China, South China, Southwest China and Central China is rich in plant resources and widely distributed. [0003] With the deepening of pharmacological research, it is found that privet plants have various physiological activities, but the research on the chemical constituents of privet plants is not enough. For a long time, the parts of the privet plant we have studied have not been fully utilized, basically not used as medic...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 司福亭李靖靖姚虹周晓莉司方博付鸿宁建中岳福兴高建炳王文武王宇飞华慧颖曾超
Owner 曾超
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