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Denitrification method by sewage short-cut simultaneous nitrification and denitrification

A technology of simultaneous nitrification and denitrification, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the problems of microbial function restriction, unstable denitrification effect, etc., and reach the scope of application Wide, improved effect, strong impact resistance effect

Active Publication Date: 2013-07-24
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

[0007] Aiming at the shortcomings of the current simultaneous nitrification and denitrification process, the present invention provides a method of directly inoculating microorganisms with similar growth conditions to realize simultaneous nitrification and denitrification, and solves the mutual constraints of microbial functions encountered in the practical application of simultaneous nitrification and denitrification. Unstable denitrification effect and other issues

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  • Denitrification method by sewage short-cut simultaneous nitrification and denitrification

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preparation example Construction

[0017] A kind of specific preparation method of the nitrous acid type denitrification microbial bacterial agent that the inventive method uses comprises the following content:

[0018] 1. Arthrobacter FDN-1, Flavobacterium waterii FDN-2, Paracoccus nitrogenus DN-3 and Methylobacterium SDN-3 of the present invention are respectively inoculated on a solid medium for activation;

[0019] 2. Use the inoculation loop to inoculate the colonies on the plate into the corresponding liquid culture solution, and culture them under aerobic conditions at a temperature of 25-35°C and 150-240 rpm for 1-3 days until the logarithmic growth phase, and obtain liquid bacteria agent seed liquid;

[0020] 3. After amplifying and culturing the above-mentioned seed liquid, the bacteria are collected, filtered and concentrated, and then mixed according to the required ratio to obtain the nitrous acid denitrification microbial bacterial agent of the present invention. The seed solution of each bacteri...

Embodiment 1

[0026] The preparation of embodiment 1 microbial bacterial agent

[0027] 1. Bacteria activation: The activation medium formula of Arthrobacter FDN-1 and Flavobacterium waterii FDN-2 is: beef extract: 5g / L, peptone: 10g / L, NaNO 2 : 1g / L, add 2.0% agar; the activation medium formula of Paracoccus nitrogen DN-3 is: KNO 3 : 1g / L, sodium succinate: 8g / L, KH 2 PO 4 : 1g / L, FeCL 2 : 0.5g / L, add 2.0% agar; the activation medium formula of Methylobacterium SDN-3 is: ammonium sulfate: 0.5g / L, methanol: 0.75mL / L, KH 2 PO 4 : 1g / L, FeCL 2 : 0.5g / L,; add 2% agar. After spreading evenly on the plate, place it in a constant temperature incubator at a temperature of 30°C for activation.

[0028] 2. Use the inoculation loop to scrape the bacteria on the plate and inoculate them in the corresponding liquid culture solution respectively, and culture them with shaking under aerobic conditions of 25-35°C and 150-240rpm for 1-3 days until the logarithmic growth phase, and obtain Liquid bac...

Embodiment 2

[0031] Get the bacterium suspension A that a certain amount of embodiment 1 obtains and inoculate in two aeration reactors according to the inoculum size of 5% (volume), at 30 ℃ of temperature, pH 7.7, DO (dissolved oxygen) is 0.9~2.9mg. L -1 Under the conditions, the treatment of ammonia-containing wastewater generated in the urea production process, NH 3 -The average concentration of N is 450mg / L, the average concentration of total nitrogen (TN) is 500mg / L, COD Cr The average concentration is 150mg / L. During the treatment process, methanol is supplemented in No. 1 reactor as a carbon source, and no carbon source is supplemented in No. 2 reactor. After 18 hours of reaction, the ammonia nitrogen removal rate of the two reactors was greater than 90%, and the total nitrogen removal rate of No. 1 reactor with sufficient carbon source was 90%. During the reaction, the molar ratio of nitrite to nitrate was greater than 9:1, Simultaneous short-range nitrification and denitrificati...

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Abstract

The invention discloses a denitrification method by sewage short-cut simultaneous nitrification and denitrification, comprising the following step of: adding a nitrous acid denitrifying inoculant into sewage containing ammonia and nitrogen, wherein the ammonia-nitrogen-containing sewage treatment temperature is 18-40 DEG C, dissolved oxygen is 0.1-3mg / L, and pH is 7-9. According to the method, the heterotrophic nitrobacteria are used for advantage combination as the enhanced microorganism to process wastewater, thus realizing the removal of ammonia nitrogen, total nitrogen and CODcr in a samereactor. The effect of wastewater treatment is good. The method provided by the invention is especially suitable for the purification treatment of wastewater containing ammonia nitrogen, and can be used to actually realize denitrification by short-cut nitrification and denitrification or simultaneous nitrification and denitrification.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering sewage biological treatment, in particular relates to a waste water treatment method mainly used for treating ammonia-containing waste water, in particular to a waste water treatment method capable of realizing short-range synchronous nitrification, denitrification and denitrification. Background technique [0002] Simultaneous nitrification and denitrification (SND) means that the nitrification and denitrification reactions are carried out simultaneously in the same reactor. This new denitrification process not only overcomes the shortcomings of the traditional biological denitrification process of nitrification and denitrification in two different reactors or sequentially in the same reactor (SBR), but also reduces energy and material consumption. etc. have outstanding advantages, especially the SND process with nitrite nitrogen has more obvious advantages. For example, the ox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/30C02F3/34
Inventor 高会杰黎元生李志瑞孙丹凤刘忠生
Owner CHINA PETROLEUM & CHEM CORP
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