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Zymotic fluid for biofermentation and preparation method thereof

A technology for biological fermentation and fermentation broth, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high preparation cost and single nutrient, and achieve safe and stable quality, high nutrient yield, The effect of low manufacturing cost

Active Publication Date: 2012-05-16
上海玉臻健康科学研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its preparation cost is high and its nutrients are single. In order to overcome these problems, an industrial-grade domesticated fermentation broth preparation method is now needed, which can obtain a variety of nutrients, safety, and stability through low-cost fermentation. fermentation broth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] A method for preparing a fermented liquid for biological fermentation, the method comprising the following steps:

[0063] 1. Ingredients and dosage of raw materials and auxiliary materials (according to the percentage of ingredients)

[0064] Sake yeast liquid: 0.5% (bacteria content 4×10 10 ), Streptomyces griseus solution: 0.5% (bacteria content 4×10 10 ), lactic acid bacteria solution 0.5% (bacteria content 4×10 10 ), rice distiller's grains 30%, fruit, rice, soybean mixture 20%, pure water 48.5%.

[0065] 2. Culture medium composition

[0066] (1) Sake yeast medium (pH natural): peptone 5g, glucose 10g, yeast extract 3g, wort 1000ml;

[0067] (2) Streptococcus griseus culture medium (pH7.2): Potassium nitrate 1g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium chloride 0.5g, ferrous sulfate 0.0075g, soluble starch 10g, pure water 1000ml ;

[0068](3) Lactic acid bacteria culture medium (natural pH): 10g of milk casein peptone, 10g of pepto...

Embodiment 2

[0086] A method for preparing a fermented liquid for biological fermentation, the method comprising the following steps:

[0087] (1) Prepare raw materials: 0.3kg of sake yeast liquid, 0.3kg of streptococcus griseus liquid, 0.3kg of lactic acid bacteria liquid, 30kg of rice distiller's grains, 10kg of rice, 59.1kg of pure water,

[0088] Wherein, the preparation method of sake yeast liquid comprises the following steps:

[0089] a. Prepare sake yeast medium raw materials, including the following components and parts by weight: peptone 4g, glucose 8g, yeast extract 2g, wort juice 900g;

[0090] b. Preparation of liquid medium: Prepare raw materials according to the formula of sake yeast medium, sterilize at 0.15MPa, 121° C. for 30 minutes, obtain sterilized liquid medium, and place it in a fermenter;

[0091] c. Preparation of strains in shake flasks: Take sake yeast strains preserved on an inclined plane and insert them into sterilized liquid medium in a sterile environment, ...

Embodiment 3

[0105] A method for preparing a fermented liquid for biological fermentation, the method comprising the following steps:

[0106] (1) Preparation of raw materials: 0.8 kg of sake yeast liquid, 0.8 kg of streptococcus griseus liquid, 0.8 kg of lactic acid bacteria liquid, 50 kg of rice distiller's grains, 10 kg of rice, 20 kg of soybeans, 17.6 kg of pure water,

[0107] Wherein, the preparation method of sake yeast liquid comprises the following steps:

[0108] a. Prepare sake yeast medium raw materials, including the following components and parts by weight: peptone 6g, glucose 12g, yeast extract 4g, wort juice 1200g;

[0109] b. Preparation of liquid medium: Prepare raw materials according to the formula of sake yeast medium, sterilize at 0.15MPa, 121° C. for 30 minutes, obtain sterilized liquid medium, and place it in a fermenter;

[0110] c. Preparation of strains in shake flasks: take sake yeast strains preserved on a slant and insert them into sterilized liquid medium in...

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Abstract

The invention relates to a zymotic fluid for biofermentation and a preparation method thereof. The zymotic fluid comprises the following components in percentage by weight: 0.3-0.8 percent of a saccharomyces sake liquid, 0.3-0.8 percent of a streptomyces griseus liquid, 0.3-0.8 percent of a lactobacillus liquid, 30-50 percent of rice wine distiller's grains, 10-30 percent of biomasses and the balance of purified water. A product can be obtained by performing static biological liquid fermentation on the components. Compared with the prior art, the zymotic fluid has the advantages of low preparation cost, high nutrient yield, extremely rich components, high stability, capability of serving as a bio-fermentation food, full replacement of a chemical synthesis nutritional additive in the conventional cosmetics by adding into skin care products, relatively low preparation cost, safe and stable quality, and fundamental solving of the problems of small quantity of nutritional substances, single or mixed components and poor stability existing in skin care products.

Description

technical field [0001] The invention relates to a fermentation liquid and a preparation method thereof, in particular to a fermentation liquid for biological fermentation and a preparation method thereof. Background technique [0002] The principle of the existing biological fermentation process is mainly to use the method of microbial culture, select a single strain and put it into the fermentation substrate to make a fermentation liquid with a single nutritional component. However, its preparation cost is high and its nutrients are single. In order to overcome these problems, an industrial-grade domesticated fermentation broth preparation method is now needed, which can obtain a variety of nutrients, safety, and stability through low-cost fermentation. fermentation broth. Contents of the invention [0003] The object of the present invention is to provide a fermented liquid for biological fermentation that is relatively cheap in production cost, safe and stable in quali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/16C12N1/20C12R1/645C12R1/545C12R1/225
Inventor 冯坤范
Owner 上海玉臻健康科学研究有限公司
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