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Chemical activity of natural compound, and use of natural compound

A technology of natural compounds and medicines, applied in the field of chemical activity of natural medicines

Inactive Publication Date: 2012-05-09
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] There are currently no reports on the chemical activity of this natural compound (Lob)

Method used

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  • Chemical activity of natural compound, and use of natural compound
  • Chemical activity of natural compound, and use of natural compound
  • Chemical activity of natural compound, and use of natural compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Animal experiments

[0040] 1.1 Experimental animals

[0041] SD rats (250-300g), half male and half male, provided by Dongchuang Experimental Animal Science and Technology Service Department, Kaifu District, Changsha City, animal license number: SCXK (Xiang) 2009-0012.

[0042] After all the animals arrived, they were quarantined in the quarantine room for 3 days and then moved to the animal breeding room. All rats were reared 5 / cage, separated from male and female, with normal diet and free access to water. The temperature in the animal breeding room is 20-25°C, the daily temperature difference does not exceed 3°C, the relative humidity is 40-70%, the working illumination is 150-300lx, the animal illumination is 15-20lx, and the day and night light is 12 / 12h alternately.

[0043] 1.2 Reagents and instruments

[0044] 1.2.1 Reagents

[0045] Table 1 Experimental Reagents

[0046]

[0047]

[0048] 1.2.2 Instruments

[0049] Table 2 Experimental Instrumen...

Embodiment 2

[0123] 2. Cell experiments

[0124] 2.1 Cell lines

[0125] H9C2(2-1) rat cardiomyocyte cell line was purchased from Shanghai Institute of Biological Sciences, Chinese Academy of Sciences.

[0126] 2.2 Reagents and instruments

[0127] 2.2.1 Reagents

[0128] Table 4 Experimental Reagents

[0129]

[0130] 2.2.2 Instruments

[0131]Table 5 Experimental Instruments

[0132] CO 2 cell culture incubator

USA THERMO

DMIL Inverted Phase Contrast Microscope

Germany

fluorescence microscope

Germany

Desktop low speed centrifuge

Hunan Kaida Industrial Co., Ltd.

biological safety cabinet

Shanghai Ruiyang

Medical clean bench

Suzhou Feng's

UV-Vis Spectrophotometer

Beijing Labtech Instrument Co., Ltd.

Hitachi 7180 Model Automatic Biochemical Analyzer

Hitachi

MR-96A microplate reader

Shenzhen Mindray

[0133] MW-12A plate washer

Shenzhen Mindray

PTC0200 PCR...

Embodiment 3

[0209] Embodiment 3: (the result of embodiment 1 and 2)

[0210] 3. Results

[0211] 3.1 Animal experiments

[0212] 3.1.1 Effect of LOB on myocardial infarct size in rats

[0213] like image 3 As shown, TTC staining in myocardial tissue is white staining, which is the infarct area. Myocardium tissue in the normal blank control group was red-stained, and there was no infarction; the myocardial tissue in the sham operation group underwent surgery but did not ligate the coronary artery, and the myocardial tissue was also red-stained, suggesting that the operation had no significant effect on the myocardium; the myocardial tissue in the ischemia-reperfusion model group Part of the infarct area can be seen in the section, which is 32.1±3.5% of the total area; and different doses of LOB injected into the sublingual vein 10 minutes before ischemia can reduce the myocardial infarct area induced by ischemia-reperfusion injury to varying degrees, among which the medium and high Th...

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PUM

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Abstract

The invention belongs to the field of natural pharmaceutical chemistry, specifically relates to a chemical activity of a natural drug, and a use of the natural drug. The structural formula of the natural compound is represented by the formula (1), and Lob is the abbreviation for the natural compound in the instruction. The present invention provides an application of the natural compound (Lob) infields of myocardial ischemia reperfusion injury protection and anoxic and re-oxygenation injury protection. In addition, the present invention further provides an application of the natural compound(Lob) in preparation of drugs for treatment of myocardial infarction.

Description

technical field [0001] The invention belongs to the field of natural medicine chemistry, and in particular relates to the chemical activity of a natural medicine and the application of the natural medicine. Background technique [0002] A natural compound: the chemical structural formula is: [0003] [0004] Chemical name: Chrysoeriol 7-O-[β-D-glucuronopyranosyl-(1→2)-O-β-D-glucuronopyranoside] [0005] Molecular formula: C 28 h 28 o 18 [0006] At present, there is no accurate Chinese name for the natural compound. For the convenience of description, the natural compound is abbreviated as: Lob in the present invention. [0007] The natural compound (Lob) is extracted and separated from the traditional Chinese medicine lobelia, the structure is identified by UV, IR, NMR and MS, and the purity detected by HPLC normalization method is greater than 98.5%. [0008] Extraction and separation process: Lobelia dried medicinal materials, 10 times the amount of 70% ethanol ...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P9/10
Inventor 徐康平张国刚邹辉张凯李福双谭桂山
Owner CENT SOUTH UNIV
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