Method for increasing transplanting success rate of tissue culture plantlets of honeysuckle

Abstract

A method for increasing transplanting success rate of tissue culture plantlets of honeysuckle enables honeysuckle plants to gradually adapt to external environment by means of seedling hardening, transplanting, recovery-phase management, growth-phase management and outplanting management, so that the honeysuckle plants correspondingly vary in physiology, form and organization to be more adaptive to the nature, the success rate of the tissue culture plantlets of honeysuckle is increased greatly, and the plants grow healthily and well.

Description

technical field [0001] The invention relates to a plant tissue culture rapid propagation technology, in particular to a method for transplanting honeysuckle tissue culture seedlings. Background technique [0002] Honeysuckle belongs to Lonicera, and the whole plant can be used as medicine. The buds contain volatile aromatic oils, inositol, saponins, chlorogenic acid, flavonoids, cinnamic acid, nerolidol, jasmonal, etc. The stems contain alkaloids. It contains honeysuckle and luteolin, etc. It is one of the traditional Chinese medicinal materials, and it is also a raw material for both medicine and food. It has the effects of clearing away heat and detoxifying, cooling wind and heat, protecting the liver and promoting gallbladder. It has been used in the field of traditional Chinese medicine for carbuncles and boils. , sore throat, erysipelas, heat-toxin bloody dysentery, wind-heat cold, and treatment of warm-heat diseases; now it is more used for health care and health tea, ...

AI Technical Summary

Problems solved by technology

At present, honeysuckle is mainly propagated by traditional methods such as cuttings, sowing, grafting, and layering, which are not only limited by seasons, but also have a low survival rate and cannot meet the needs of large-scale production; although grafting propagation has the advantages of strong plant adaptability and well-developed root system, However, a large number of wild resources must be collected, and the process is complicated, and the survival rate is also very low.

Only through tissue culture and propagation can large quantities of honeysuckle seedlings be rapidly propagated, but the honeysuckle seedlings that have undergone tissue culture grow under sterile, nutrient supply, suitable light and a relative humidity of nearly 100% of the temperature. Therefore, in Seedlings grown under natural conditions are very different in terms of physiology and shape. When they are transplanted into greenhouses, the rate of water loss will be high and the seedlings will die easily.